Studying the interaction of neutrophils and glaesserella parasuis indicates a serotype independent benefit from degradation of NETs
Glaesserella (G.) parasuis is one of the most important porcine pathogens causing Glaesser’s disease. Neutrophil granulocytes are the major counteracting cell type of the innate immune system, which contribute to the host defense by phagocytosis or the formation of neutrophil extracellular traps...
| Autores Principales: | Bonilla, Marta C., Lassnig, Simon, Obando Corella , Andrea, Imker, Rabea, Valentin-Weigand, Peter, von Köckritz-Blickwede, Maren, Luther, Anne-Marie, Hennig-Pauka, Isabel, de Buhr, Nicole |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
MDPI
2022
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| Materias: | |
| Acceso en línea: |
https://www.mdpi.com/journal/pathogens http://hdl.handle.net/11056/24319 https://doi.org/10.3390/pathogens11080880 |
| Sumario: |
Glaesserella (G.) parasuis is one of the most important porcine pathogens causing Glaesser’s
disease. Neutrophil granulocytes are the major counteracting cell type of the innate immune system,
which contribute to the host defense by phagocytosis or the formation of neutrophil extracellular
traps (NETs). Recently, NET-formation has been shown to facilitate the survival of bacteria from
the Pasteurellaceae family. However, the interaction of NETs and G. parasuis is unclear so far. In this
study, we investigated the interplay of three G. parasuis serotypes with porcine neutrophils. The
production of reactive oxygen species by neutrophils after G. parasuis infection varied slightly among
the serotypes but was generally low and not significantly influenced by the serotypes. Interestingly,
we detected that independent of the serotype of G. parasuis, NET formation in neutrophils was
induced to a small but significant extent. This phenomenon occurred despite the ability of
G. parasuis to release nucleases, which can degrade NETs. Furthermore, the growth of Glaesserella was
enhanced by external DNases and degraded NETs. This indicates that Glaesserella takes up degraded
NET components, supplying them with nicotinamide adenine dinucleotide (NAD), as this benefit
was diminished by inhibiting the 50
-nucleotidase, which metabolizes NAD. Our results indicate
a serotype-independent interaction of Glaesserella with neutrophils by inducing NET-formation and
benefiting from DNA degradation. |
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