Comparison of competitive enzyme-linked immunosorbent assay and serum neutralization test for the detection of antibodies to vesicular stomatitis virus (New Jersey and Indiana serotypes) in equines from Costa Rica
A competitive enzyme-linked immunosorbent assay (c-ELISA) was compared to the serum neutralization test (SNT) for the detection of antibodies against vesicular stomatitis virus, New Jersey (VSV-NJ) and Indiana (VSV-IN) serotypes, using 214 equine serum samples from Costa Rica. In the c-ELISA for...
Autores Principales: | Dolz, Gaby, von Köckritz-Blickwede, Maren, Herrero-Acosta, Marco Vinicio |
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Formato: | Artículo |
Idioma: | Inglés |
Publicado: |
Universidad Nacional, Costa Rica
2020
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Materias: | |
Acceso en línea: |
http://hdl.handle.net/11056/17804 |
Sumario: |
A competitive enzyme-linked immunosorbent assay (c-ELISA) was compared to the serum
neutralization test (SNT) for the detection of antibodies against vesicular stomatitis virus, New Jersey
(VSV-NJ) and Indiana (VSV-IN) serotypes, using 214 equine serum samples from Costa Rica. In the
c-ELISA for VSV-NJ 109 (50.93%) equine sera reacted positive, whereas in the SNT 138 (64.48%)
serum samples reacted positive to VSV-NJ. In the c-ELISA for VSV-IN 26 (12.15%) sera reacted
positive, whereas in the SNT 36 (16.83%) equine sera reacted positive to VSV-IN. The c-ELISA showed
a moderate sensitivity compared to the SNT. The rapidity and ease of the c-ELISA system makes
it suitable as a rapid diagnostic screening assay. However, because of the lower sensitivity of the
c-ELISA, the SNT is considered the method of choice to detect equine VSV infections in Costa Rica. |
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