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Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies

An indirect enzyme-linked immunosorbent assay (ELISA) for the diagnosis of bovine leukaemia virus (BLV) infection was developed and compared with the agar gel immunodiffusion test (AGIDT). Western blotting (WB) was used as confirmatory test. ELISA and AGIDT had specificities that were comparable wit...

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Autores Principales: Dolz, Gaby, Moreno, Edgardo
Formato: Artículo
Idioma: Inglés
Publicado: Blackwell 2022
Materias:
LEUCOSIS BOVINA
VIRUSES
VIRUS
BLV
GANADO BOVINO
BOVINE
Acceso en línea: http://hdl.handle.net/11056/23002
https://doi.org/10.1111/j.1439-0450.1999.tb01248.x
id RepoUNACR23002
recordtype dspace
spelling RepoUNACR230022022-05-07T00:13:50Z Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies Dolz, Gaby Moreno, Edgardo LEUCOSIS BOVINA VIRUSES VIRUS BLV GANADO BOVINO BOVINE An indirect enzyme-linked immunosorbent assay (ELISA) for the diagnosis of bovine leukaemia virus (BLV) infection was developed and compared with the agar gel immunodiffusion test (AGIDT). Western blotting (WB) was used as confirmatory test. ELISA and AGIDT had specificities that were comparable with that of WB, however, ELISA showed a higher sensitivity than AGIDT. The ELISA was useful for screening a large number of samples, whereas WB was important for detecting the antibody response against the individual BLV-proteins. Different types of positive serological reactions were discerned in WB, that correlated with reactions of sera in AGIDT and ELISA. The most important antigen in WB and ELISA was the BLV protein p24, whereas the BLV glycoproteins gp51 and gp30 were of special importance in AGIDT. The relevance of repeatedly testing the antibody response in BLV-infected herds for control and eradication programmes using assays with higher sensitivity than AGIDT was demonstrated. Se elaboró un ensayo inmunoenzimático indirecto (ELISA) para el diagnóstico de la infección por el virus de la leucemia bovina (VLB) y se comparó con la prueba de inmunodifusión en gel de agar (AGIDT). La prueba de Western blot (WB) se utilizó como prueba de confirmación. La ELISA y la AGIDT tenían especificidades comparables a la de la WB, aunque la ELISA mostró una mayor sensibilidad que la AGIDT. El ELISA fue útil para el cribado de un gran número de muestras, mientras que el WB fue importante para detectar la respuesta de los anticuerpos contra las proteínas individuales del BLV. Se detectaron diferentes tipos de reacciones serológicas positivas en WB, que se correlacionaron con las reacciones de los sueros en AGIDT y ELISA. El antígeno más importante en WB y ELISA fue la proteína p24 del VLB, mientras que las glicoproteínas gp51 y gp30 del VLB fueron de especial importancia en AGIDT. Se demostró la pertinencia de analizar repetidamente la respuesta de los anticuerpos en rebaños infectados por el VLB para los programas de control y erradicación, utilizando ensayos de mayor sensibilidad que el AGIDT. Universidad Nacional, Costa Rica Escuela de Medicina Veterinaria 2022-05-07T00:12:10Z 2022-05-07T00:12:10Z 1999-10 http://purl.org/coar/resource_type/c_6501 http://hdl.handle.net/11056/23002 https://doi.org/10.1111/j.1439-0450.1999.tb01248.x eng Acceso abierto Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Blackwell J. Vet. Med. B Vol. 46 no. 8 : 551-558,1999
institution Universidad Nacional de Costa Rica
collection Repositorio UNA-Costa Rica
language Inglés
topic LEUCOSIS BOVINA
VIRUSES
VIRUS
BLV
GANADO BOVINO
BOVINE
spellingShingle LEUCOSIS BOVINA
VIRUSES
VIRUS
BLV
GANADO BOVINO
BOVINE
Dolz, Gaby
Moreno, Edgardo
Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies
description An indirect enzyme-linked immunosorbent assay (ELISA) for the diagnosis of bovine leukaemia virus (BLV) infection was developed and compared with the agar gel immunodiffusion test (AGIDT). Western blotting (WB) was used as confirmatory test. ELISA and AGIDT had specificities that were comparable with that of WB, however, ELISA showed a higher sensitivity than AGIDT. The ELISA was useful for screening a large number of samples, whereas WB was important for detecting the antibody response against the individual BLV-proteins. Different types of positive serological reactions were discerned in WB, that correlated with reactions of sera in AGIDT and ELISA. The most important antigen in WB and ELISA was the BLV protein p24, whereas the BLV glycoproteins gp51 and gp30 were of special importance in AGIDT. The relevance of repeatedly testing the antibody response in BLV-infected herds for control and eradication programmes using assays with higher sensitivity than AGIDT was demonstrated.
format Artículo
author Dolz, Gaby
Moreno, Edgardo
author_sort Dolz, Gaby
title Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies
title_short Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies
title_full Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies
title_fullStr Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies
title_full_unstemmed Comparison of Agar Gel Immunodiffusion Test, Enzyme-linked Immunosorbent Assay and Western Blotting for the Detection of BLV Antibodies
title_sort comparison of agar gel immunodiffusion test, enzyme-linked immunosorbent assay and western blotting for the detection of blv antibodies
publisher Blackwell
publishDate 2022
url http://hdl.handle.net/11056/23002
https://doi.org/10.1111/j.1439-0450.1999.tb01248.x
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score 12.23286

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