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Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica

Outbreaks caused by Chlamydia psittaci and other chlamydial species have recently been reported in poultry farms worldwide, causing considerable economic losses. The objective of this study was to determine the presence of chlamydial species in these birds in Costa Rica. One hundred and fifty poo...

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Autores Principales: Dolz, Gaby, Solorzano-Morales, Antony
Formato: Artículo
Idioma: Inglés
Publicado: Cambridge University Press 2022
Materias:
POLLOS
AVICULTURA
POULTRY
CHLAMYDIA
COSTA RICA
Acceso en línea: http://hdl.handle.net/11056/22830
https://doi.org/10.1017/S0950268821002715
id RepoUNACR22830
recordtype dspace
spelling RepoUNACR228302022-03-30T22:57:56Z Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica Dolz, Gaby Solorzano-Morales, Antony POLLOS AVICULTURA POULTRY CHLAMYDIA COSTA RICA Outbreaks caused by Chlamydia psittaci and other chlamydial species have recently been reported in poultry farms worldwide, causing considerable economic losses. The objective of this study was to determine the presence of chlamydial species in these birds in Costa Rica. One hundred and fifty pools of lung tissue samples from industrial poultry with respiratory problems and 112 pools of tracheal swabs from asymptomatic backyard poultry were analysed by real-time quantitative polymerase chain reaction (qPCR), end-point PCR and sequencing. A total of 16.8% (44/262) samples were positive for Chlamydia spp., most of them detected in asymptomatic backyard poultry (28.6%, 32/112) and fewer in industrial poultry (8%, 12/150). Of these positive samples, 45.5% (20/44) were determined to be C. psittaci. For the first time C. psittaci genotype A is reported in poultry in Latin America. In addition, the presence of Chlamydia gallinacea in backyard poultry and of Chlamydia muridarum in industrial and backyard poultry is reported for the first time in Central America. In 40.9% (18/44) of the positive samples, it was not possible to identify the infecting chlamydial species. These findings reveal a zoonotic risk, particularly for poultry farm and slaughterhouse workers having direct contact with these birds. Recientemente se han reportado brotes causados por Chlamydia psittaci y otras especies clamidiales en granjas avícolas de todo el mundo, causando considerables pérdidas económicas. El objetivo de este estudio fue determinar la presencia de especies clamidiales en estas aves en Costa Rica. Se analizaron ciento cincuenta pools de muestras de tejido pulmonar de aves de corral industriales con problemas respiratorios y 112 pools de hisopos traqueales de aves de corral de traspatio asintomáticas mediante reacción en cadena de la polimerasa cuantitativa en tiempo real (qPCR), PCR de punto final y secuenciación. Un 16,8% (44/262) de las muestras fueron positivas a Chlamydia spp., la mayoría de ellas detectadas en aves de corral de traspatio asintomáticas (28,6%, 32/112) y menos en aves de corral industriales (8%, 12/150). De estas muestras positivas, se determinó que el 45,5% (20/44) eran C. psittaci. Por primera vez se reporta el genotipo A de C. psittaci en aves de corral en América Latina. Además, se reporta por primera vez en Centroamérica la presencia de Chlamydia gallinacea en aves de traspatio y de Chlamydia muridarum en aves industriales y de traspatio. En el 40,9% (18/44) de las muestras positivas no fue posible identificar la especie de clamidia infectante. Estos resultados revelan un riesgo zoonótico, en particular para los trabajadores de las granjas avícolas y de los mataderos que tienen contacto directo con estas aves. FUNDAUNA Universidad Nacional, Costa Rica Escuela de Medicina Veterinaria 2022-03-30T22:53:48Z 2022-03-30T22:53:48Z 2022-03-24 http://purl.org/coar/resource_type/c_6501 1469-4409 http://hdl.handle.net/11056/22830 https://doi.org/10.1017/S0950268821002715 eng Acceso abierto http://creativecommons.org/licenses/by-nc-nd/ application/pdf Cambridge University Press Epidemiology & Infection Vol. 150, e67
institution Universidad Nacional de Costa Rica
collection Repositorio UNA-Costa Rica
language Inglés
topic POLLOS
AVICULTURA
POULTRY
CHLAMYDIA
COSTA RICA
spellingShingle POLLOS
AVICULTURA
POULTRY
CHLAMYDIA
COSTA RICA
Dolz, Gaby
Solorzano-Morales, Antony
Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica
description Outbreaks caused by Chlamydia psittaci and other chlamydial species have recently been reported in poultry farms worldwide, causing considerable economic losses. The objective of this study was to determine the presence of chlamydial species in these birds in Costa Rica. One hundred and fifty pools of lung tissue samples from industrial poultry with respiratory problems and 112 pools of tracheal swabs from asymptomatic backyard poultry were analysed by real-time quantitative polymerase chain reaction (qPCR), end-point PCR and sequencing. A total of 16.8% (44/262) samples were positive for Chlamydia spp., most of them detected in asymptomatic backyard poultry (28.6%, 32/112) and fewer in industrial poultry (8%, 12/150). Of these positive samples, 45.5% (20/44) were determined to be C. psittaci. For the first time C. psittaci genotype A is reported in poultry in Latin America. In addition, the presence of Chlamydia gallinacea in backyard poultry and of Chlamydia muridarum in industrial and backyard poultry is reported for the first time in Central America. In 40.9% (18/44) of the positive samples, it was not possible to identify the infecting chlamydial species. These findings reveal a zoonotic risk, particularly for poultry farm and slaughterhouse workers having direct contact with these birds.
format Artículo
author Dolz, Gaby
Solorzano-Morales, Antony
author_sort Dolz, Gaby
title Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica
title_short Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica
title_full Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica
title_fullStr Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica
title_full_unstemmed Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica
title_sort molecular characterization of chlamydia species in commercial and backyard poultry farms in costa rica
publisher Cambridge University Press
publishDate 2022
url http://hdl.handle.net/11056/22830
https://doi.org/10.1017/S0950268821002715
_version_ 1796094815250677760
score 12.2319145

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