Molecular characterization of Chlamydia species in commercial and backyard poultry farms in Costa Rica
Outbreaks caused by Chlamydia psittaci and other chlamydial species have recently been reported in poultry farms worldwide, causing considerable economic losses. The objective of this study was to determine the presence of chlamydial species in these birds in Costa Rica. One hundred and fifty poo...
| Autores Principales: | Dolz, Gaby, Solorzano-Morales, Antony |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
Cambridge University Press
2022
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| Materias: | |
| Acceso en línea: |
http://hdl.handle.net/11056/22830 https://doi.org/10.1017/S0950268821002715 |
| Sumario: |
Outbreaks caused by Chlamydia psittaci and other chlamydial species have recently been
reported in poultry farms worldwide, causing considerable economic losses. The objective
of this study was to determine the presence of chlamydial species in these birds in Costa
Rica. One hundred and fifty pools of lung tissue samples from industrial poultry with respiratory
problems and 112 pools of tracheal swabs from asymptomatic backyard poultry were analysed
by real-time quantitative polymerase chain reaction (qPCR), end-point PCR and
sequencing. A total of 16.8% (44/262) samples were positive for Chlamydia spp., most of
them detected in asymptomatic backyard poultry (28.6%, 32/112) and fewer in industrial
poultry (8%, 12/150). Of these positive samples, 45.5% (20/44) were determined to be C. psittaci.
For the first time C. psittaci genotype A is reported in poultry in Latin America. In addition,
the presence of Chlamydia gallinacea in backyard poultry and of Chlamydia muridarum
in industrial and backyard poultry is reported for the first time in Central America. In 40.9%
(18/44) of the positive samples, it was not possible to identify the infecting chlamydial species.
These findings reveal a zoonotic risk, particularly for poultry farm and slaughterhouse workers
having direct contact with these birds. |
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