A Selective Ion HPLC-APCI-MS Method for the Quantification of Pentacyclic Triterpenes in an Anxiolytic Botanical Dietary Supplement for the Animal Health Market
A new anti-anxiety dietary supplement was developed for the animal health market, by combining 2 triterpene containing botanicals, Souroubea sympetala (Marcgraviaceae) with Platanus occidentalis (Platanaceae) A validated method for a quality control of the botanical blend was achieved using HPLC-AP...
| Autores Principales: | Liu, Rui, Carballo Arce, Ana Francis, Singh, Ranpreet, Saleem, Ammar, Rocha, Marco, Mullally, Martha, Otarola-Rojas, Marco, Poveda Alvarrez, Luis, Sanchez-Vindas, Pablo, Garcia, Mario, Baker, John, Merali, Zul, Guerrero-Analco, José A., Durst, Tony, Harris, Cory, Arnason, John |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
Natural Product Incorporation
2022
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| Materias: | |
| Acceso en línea: |
http://hdl.handle.net/11056/22332 |
| Sumario: |
A new anti-anxiety dietary supplement was developed for the animal health market, by combining 2 triterpene containing botanicals, Souroubea sympetala
(Marcgraviaceae) with Platanus occidentalis (Platanaceae) A validated method for a quality control of the botanical blend was achieved using HPLC-APCIMS. The method resulted in the detection and quantitative determination of betulinic acid (1), and ursolic acid (2) in P. occidentalis and 1, 2, lupeol (3),
β-amyrin (4) and α-amyrin (5) in S. sympetala and the finished product ZentrolTM. Detections were at low ng on column for 1 and 2 and in low µg range for
3, 4 and 5 using calibration curves within 10-100 ng (R2
> 0.993). Recovery of spiked samples for all the recoveries observed were > 94%. Inter-day and intraday variations were 0.8-3.5% and 5-10.4%, respectively. These results indicate the suitability of the developed analytical method to detect and quantify
triterpenes of raw materials used in the manufacture of natural health products |
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