Efficacy of Souroubea-Platanus Dietary Supplement Containing Triterpenes in Beagle Dogs Using a Thunderstorm Noise-Induced Model of Fear and Anxiety
A novel botanical dietary supplement, formulated as a chewable tablet containing a defined mixture of Souroubea spp. vine and Platanus spp. Bark, was tested as a canine anxiolytic for thunderstorm noise-induced stress (noise aversion). The tablet contained five highly stable triterpenes and deliv...
| Autores Principales: | Masic, Aleksandar, Landsberg, Gary, Milgram, Bill., Merali, Zul., Durst, Tony, Sánchez Vindas, Pablo, Garcia, Mario, Baker, John, Liu, Rui, Arnason, John |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
Multidisciplinary Digital Publishing Institute
2021
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| Materias: | |
| Acceso en línea: |
http://hdl.handle.net/11056/21934 https://doi.org/10.3390/molecules26072049 |
| Sumario: |
A novel botanical dietary supplement, formulated as a chewable tablet containing a
defined mixture of Souroubea spp. vine and Platanus spp. Bark, was tested as a canine anxiolytic
for thunderstorm noise-induced stress (noise aversion). The tablet contained five highly stable
triterpenes and delivered 10 mg of the active ingredient betulinic acid (BA) for an intended 1 mg/kg
dose in a 10 kg dog. BA in tablets was stable for 30 months in storage at 23 ◦C. Efficacy of the tablets
in reducing anxiety in dogs was assessed in a blinded, placebo-controlled study by recording changes
in blood cortisol levels and measures of behavioral activity in response to recorded intermittent
thunder. Sixty beagles were assigned into groups receiving: placebo, 0.5×, 1×, 2×, and 4× dose, or
the positive control (diazepam), for five days. Reduction in anxiety measures was partially dosedependent and the 1× dose was effective in reducing inactivity time (p = 0.0111) or increased activity
time (p = 0.0299) compared with placebo, indicating a decrease in anxiety response. Cortisol measures
also showed a dose-dependent reduction in cortisol in dogs treated with the test tablet. |
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