Tracking dipeptides at work-uptake and intracellular fate in CHO culture
Artículo científico
| Autores Principales: | Sánchez-Kopper, Andres, Becker, Max, Pfizenmaier, Jennifer, Kessler, Christian, Karau, Andreas, Takors, Ralf |
|---|---|
| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
AMB Express
2018
|
| Materias: | |
| Acceso en línea: |
https://amb-express.springeropen.com/articles/10.1186/s13568-016-0221-0 https://hdl.handle.net/2238/9850 |
| id |
RepoTEC9850 |
|---|---|
| recordtype |
dspace |
| spelling |
RepoTEC98502022-04-09T03:05:49Z Tracking dipeptides at work-uptake and intracellular fate in CHO culture Sánchez-Kopper, Andres Becker, Max Pfizenmaier, Jennifer Kessler, Christian Karau, Andreas Takors, Ralf Anticuerpos Células Optimización Producción Aminoácidos Research Subject Categories::NATURAL SCIENCES::Chemistry Artículo científico Market demands for monoclonal antibodies (mAbs) are steadily increasing worldwide. As a result, production processes using Chinese hamster ovary cells (CHO) are in the focus of ongoing intensification studies for maximizing cell-specific and volumetric productivities. This includes the optimization of animal-derived component free (ADCF) cultivation media as part of good cell culture practice. Dipeptides are known to improve CHO culture performance. However, little or even conflicting assumptions exist about their putative import and functionality inside the cells. A set of well-known performance boosters and new dipeptide prospects was evaluated. The present study revealed that dipeptides are indeed imported in the cells, where they are decomposed to the amino acids building blocks. Subsequently, they are metabolized or, unexpectedly, secreted to the medium. Monoclonal antibody production boosting additives like l-alanine-l-glutamine (AQ) or glycyl-l-glutamine (GQ) can be assigned to fast or slow dipeptide uptake, respectively, thus pinpointing to the need to study dipeptide kinetics and to adjust their feeding individually for optimizing mAb production. Instituto Tecnológico de Costa Rica. Escuela de Química 2018-08-03T20:24:26Z 2018-08-03T20:24:26Z 2016 info:eu-repo/semantics/article https://amb-express.springeropen.com/articles/10.1186/s13568-016-0221-0 Tracking dipeptides at work-uptake and intracellular fate in CHO culture. AMB Express, 6(1), 1-12. https://hdl.handle.net/2238/9850 eng 10.1186/s13568-016-0221-0 Attribution-NonCommercial-ShareAlike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf AMB Express AMB Express |
| institution |
Tecnológico de Costa Rica |
| collection |
Repositorio TEC |
| language |
Inglés |
| topic |
Anticuerpos Células Optimización Producción Aminoácidos Research Subject Categories::NATURAL SCIENCES::Chemistry |
| spellingShingle |
Anticuerpos Células Optimización Producción Aminoácidos Research Subject Categories::NATURAL SCIENCES::Chemistry Sánchez-Kopper, Andres Becker, Max Pfizenmaier, Jennifer Kessler, Christian Karau, Andreas Takors, Ralf Tracking dipeptides at work-uptake and intracellular fate in CHO culture |
| description |
Artículo científico |
| format |
Artículo |
| author |
Sánchez-Kopper, Andres Becker, Max Pfizenmaier, Jennifer Kessler, Christian Karau, Andreas Takors, Ralf |
| author_sort |
Sánchez-Kopper, Andres |
| title |
Tracking dipeptides at work-uptake and intracellular fate in CHO culture |
| title_short |
Tracking dipeptides at work-uptake and intracellular fate in CHO culture |
| title_full |
Tracking dipeptides at work-uptake and intracellular fate in CHO culture |
| title_fullStr |
Tracking dipeptides at work-uptake and intracellular fate in CHO culture |
| title_full_unstemmed |
Tracking dipeptides at work-uptake and intracellular fate in CHO culture |
| title_sort |
tracking dipeptides at work-uptake and intracellular fate in cho culture |
| publisher |
AMB Express |
| publishDate |
2018 |
| url |
https://amb-express.springeropen.com/articles/10.1186/s13568-016-0221-0 https://hdl.handle.net/2238/9850 |
| _version_ |
1796142381112754176 |
| score |
12.233033 |