First Report of Campylobacter hepaticus Isolation in Laying Hens and Broiler Breeders with Spotty Liver Disease in Costa Rica
Poultry producers in Costa Rica have informally reported a spotty liver disease–like syndrome for more than 20 yr. However, despite many attempts, the infectious agent responsible for this syndrome had not been identified. Therefore, following current knowledge of spotty liver disease diagnosis, w...
Autores Principales: | Quesada-Vásquez, Dioney, Jimenez-Madrigal, Laura, Chaves-Hernandez, Aida Jeannette, MUNOZ VARGAS, LOHENDY, BARQUERO-CALVO, ELIAS |
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Formato: | Artículo |
Idioma: | Español |
Publicado: |
American Association of Avian Pathologists
2023
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Materias: | |
Acceso en línea: |
http://hdl.handle.net/11056/25205 https://doi.org/10.1637/aviandiseases-D-22-00046 |
Sumario: |
Poultry producers in Costa Rica have informally reported a spotty liver disease–like syndrome for more than 20 yr.
However, despite many attempts, the infectious agent responsible for this syndrome had not been identified. Therefore, following
current knowledge of spotty liver disease diagnosis, we invited veterinarians and poultry producers to submit samples to the
diagnostic laboratories of the Veterinary Medicine School, Universidad Nacional, to identify the infectious agent of this syndrome.
Veterinarians and poultry producers were instructed to collect gallbladders and livers aseptically and send them for pathology
examinations and bacterial cultures in less than 24 hr after collection. Samples were processed for standard histopathologic studies
and cultured under aerophilic, anaerobic, and microaerophilic conditions. Campylobacter-like colonies were isolated and identified
by biochemical and PCR tests. Here we report for the first time the isolation, biochemical characterization, and molecular
confirmation of Campylobacter hepaticus in laying hens and broiler breeders with spotty liver disease in Costa Rica. |
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