Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes
Brucella abortus is an intracellular pathogen that replicates within a membrane-bounded compartment. In this study, we have examined the intracellular pathway of the virulent B. abortus strain 2308 (S2308) and the attenuated strain 19 (S19) in HeLa cells. At 10 min after inoculation, both bacteria...
| Autores Principales: | PIZARRO CERDA, JAVIER, MERESSE, STEPHANE, PARTON, ROBERT, GISOU, VAN DER, SOLA LANDA, ALBERTO, LOPEZ GOÑI, IGNACIO, GORVEL, JEAN PIERRE, Moreno, Edgardo |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
American Society for Microbiology
2022
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| Materias: | |
| Acceso en línea: |
http://hdl.handle.net/11056/22949 https://doi.org/10.1128/IAI.66.12.5711-5724.1998 |
| Sumario: |
Brucella abortus is an intracellular pathogen that replicates within a membrane-bounded compartment. In
this study, we have examined the intracellular pathway of the virulent B. abortus strain 2308 (S2308) and the
attenuated strain 19 (S19) in HeLa cells. At 10 min after inoculation, both bacterial strains are transiently
detected in phagosomes characterized by the presence of early endosomal markers such as the early endosomal
antigen 1. At ;1 h postinoculation, bacteria are located within a compartment positive for the lysosome associated membrane proteins (LAMPs) and the endoplasmic reticulum (ER) marker sec61b but negative for
the mannose 6-phosphate receptors and cathepsin D. Interestingly, this compartment is also positive for the
autophagosomal marker monodansylcadaverin, suggesting that S2308 and S19 are located in autophagic
vacuoles. At 24 h after inoculation, attenuated S19 is degraded in lysosomes, while virulent S2308 multiplies
within a LAMP- and cathepsin D-negative but sec61b- and protein disulfide isomerase-positive compartment.
Furthermore, treatment of infected cells with the pore-forming toxin aerolysin from Aeromonas hydrophila
causes vacuolation of the bacterial replication compartment. These results are compatible with the hypothesis
that pathogenic B. abortus exploits the autophagic machinery of HeLa cells to establish an intracellular niche
favorable for its replication within the ER |
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