Vista Equipo: Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes

Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes

Root-lesion nematodes of the genus Pratylenchus are among the most damaging nematodes for crop and ornamental plants worldwide. There are about 75 described species in this genus with a wide host range, but a difficult diagnosis because of morphological similarities and overlapping of morphometric c...

Descripción completa

Autores Principales:	Zamora, Tatiana, Peraza-Padilla, Walter, Archidona Yuste, Antonio, Cantalapiedra Navarrete, Carolina, Liébanas Torres, Gracia María, Palomares-Rius, Juan Emilio, Castillo, Pablo
Formato:	Artículo
Idioma:	Inglés
Publicado:	Springer 2021
Materias:	COSTA RICA MOLÉCULAS MOLECULES CULTIVOS CROPS PRATYLENCHUS MICROSCOPIA ELECTRÓNICA ELECTRONIC MICROSCOPY PANAMAENSIS BAYESIAN INFERENCE CHRYSANTHEMUM COFFEE rDNA RICE TAXONOMY TAXONOMÍA
Acceso en línea:	http://hdl.handle.net/11056/20595 https://doi.org/10.1007/s10658-016-0884-z

id	RepoUNACR20595
recordtype	dspace
spelling	RepoUNACR205952023-05-17T20:04:19Z Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes Zamora, Tatiana Peraza-Padilla, Walter Archidona Yuste, Antonio Cantalapiedra Navarrete, Carolina Liébanas Torres, Gracia María Palomares-Rius, Juan Emilio Castillo, Pablo COSTA RICA MOLÉCULAS MOLECULES CULTIVOS CROPS PRATYLENCHUS MICROSCOPIA ELECTRÓNICA ELECTRONIC MICROSCOPY PANAMAENSIS BAYESIAN INFERENCE CHRYSANTHEMUM COFFEE rDNA RICE TAXONOMY TAXONOMÍA Root-lesion nematodes of the genus Pratylenchus are among the most damaging nematodes for crop and ornamental plants worldwide. There are about 75 described species in this genus with a wide host range, but a difficult diagnosis because of morphological similarities and overlapping of morphometric characters among species. Four species of Pratylenchus were detected parasitizing cultivated and ornamental plants in Costa Rica: Pratylenchus bolivianus, P. gutierrezi, P. pseudocoffeae, and P. zeae; while P. panamaensis was detected in coffee in the type locality at Panama. The specimens were identified using morphological and molecular methods. Morphometrics and morphology using light and scanning electron microscopy are given for all of them. The presence of P. bolivianus and P. pseudocoffeae are reported for the first time in Costa Rica. The molecular and morphological variability associated with former populations of P. gutierrezi showed that we are in front of complex of species. This has been resolved with the study of topotype populations of P. gutierrezi and P. panamaensis combined with rDNA sequences (D2-D3 expansion segments of 28S and ITS). Our results, including SEM en face view data on topotypes of P. gutierrezi and P. panamaensis demonstrated that both species are almost undistinguishable morphologically, but clearly separated molecularly with D2-D3 expansion segments of 28S (89 % similar, 84 nucleotides and 19 indels) and ITS rDNA (only 36 % coverage with a 88 % similarity value). Consequently, P. gutierrezi and P. panamaensis need to be maintained as different valid taxa. Molecular data on D2-D3 expansion segments of 28S and ITS rDNA of topotypes of P. panamaensis were also coincident (99 % similarity) with root-lesion nematodes parasitizing coffee in Guatemala and Costa Rica, and banana in Madeira, and previously identified as P. gutierrezi (K1-AF170440, K2-AF170441, FR692277, respectively), which need to be considered conspecific with P. panamaensis. These results showed us the necessity of integrative taxonomy in the identification and characterization of Pratylenchus spp. Los nematodos lesionadores de raíces del género Pratylenchus se encuentran entre los nematodos más dañinos para cultivos y plantas ornamentales en todo el mundo. Hay alrededor de 75 especies descritas en este género con una amplia gama de hospedadores, pero un diagnóstico difícil debido a las similitudes morfológicas y la superposición de caracteres morfométricos entre las especies. Se detectaron cuatro especies de Pratylenchus parasitando plantas cultivadas y ornamentales en Costa Rica: Pratylenchus bolivianus, P. gutierrezi, P. pseudocoffeae y P. zeae; mientras que P. panamaensis se detectó en café en la localidad tipo de Panamá. Las muestras se identificaron mediante métodos morfológicos y moleculares. Para todos ellos se da morfometría y morfología mediante microscopía óptica y electrónica de barrido. Se reporta por primera vez en Costa Rica la presencia de P. bolivianus y P. pseudocoffeae. La variabilidad molecular y morfológica asociada a antiguas poblaciones de P. gutierrezi mostró que estamos frente a un complejo de especies. Esto se ha resuelto con el estudio de poblaciones de topotipos de P. gutierrezi y P. panamaensis combinadas con secuencias de ADNr (segmentos de expansión D2-D3 de 28S e ITS). Nuestros resultados, incluidos los datos de SEM en face view sobre topotipos de P. gutierrezi y P. panamaensis demostraron que ambas especies son casi indistinguibles morfológicamente, pero claramente separadas molecularmente con segmentos de expansión D2-D3 de 28S (89% similares, 84 nucleótidos y 19 indeles ) e ITS rDNA (solo cobertura del 36% con un valor de similitud del 88%). En consecuencia, P. gutierrezi y P. panamaensis deben mantenerse como taxones válidos diferentes. Los datos moleculares sobre los segmentos de expansión D2-D3 de 28S e ITS rDNA de topotipos de P. panamaensis también coincidieron (99% de similitud) con los nematodos lesionadores de raíces que parasitan el café en Guatemala y Costa Rica, y el banano en Madeira, y previamente identificados como P .gutierrezi (K1-AF170440, K2-AF170441, FR692277, respectivamente), que deben considerarse conespecíficas con P. panamaensis. Estos resultados nos mostraron la necesidad de una taxonomía integradora en la identificación y caracterización de Pratylenchus spp. Universidad Nacional, Costa Rica Consejo Superior de Investigaciones Científicas, España Universidad de Jaén, España Escuela de Ciencias Agrarias 2021-07-14T18:13:11Z 2021-07-14T18:13:11Z 2016-02-17 http://purl.org/coar/resource_type/c_6501 1573-8469 http://hdl.handle.net/11056/20595 https://doi.org/10.1007/s10658-016-0884-z 10.1007/s10658-016-0884-z eng Acceso abierto http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Springer European Journal of Plant Pathology, volúmen 145, páginas 973-998, 2016
institution	Universidad Nacional de Costa Rica
collection	Repositorio UNA-Costa Rica
language	Inglés
topic	COSTA RICA MOLÉCULAS MOLECULES CULTIVOS CROPS PRATYLENCHUS MICROSCOPIA ELECTRÓNICA ELECTRONIC MICROSCOPY PANAMAENSIS BAYESIAN INFERENCE CHRYSANTHEMUM COFFEE rDNA RICE TAXONOMY TAXONOMÍA
spellingShingle	COSTA RICA MOLÉCULAS MOLECULES CULTIVOS CROPS PRATYLENCHUS MICROSCOPIA ELECTRÓNICA ELECTRONIC MICROSCOPY PANAMAENSIS BAYESIAN INFERENCE CHRYSANTHEMUM COFFEE rDNA RICE TAXONOMY TAXONOMÍA Zamora, Tatiana Peraza-Padilla, Walter Archidona Yuste, Antonio Cantalapiedra Navarrete, Carolina Liébanas Torres, Gracia María Palomares-Rius, Juan Emilio Castillo, Pablo Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes
description	Root-lesion nematodes of the genus Pratylenchus are among the most damaging nematodes for crop and ornamental plants worldwide. There are about 75 described species in this genus with a wide host range, but a difficult diagnosis because of morphological similarities and overlapping of morphometric characters among species. Four species of Pratylenchus were detected parasitizing cultivated and ornamental plants in Costa Rica: Pratylenchus bolivianus, P. gutierrezi, P. pseudocoffeae, and P. zeae; while P. panamaensis was detected in coffee in the type locality at Panama. The specimens were identified using morphological and molecular methods. Morphometrics and morphology using light and scanning electron microscopy are given for all of them. The presence of P. bolivianus and P. pseudocoffeae are reported for the first time in Costa Rica. The molecular and morphological variability associated with former populations of P. gutierrezi showed that we are in front of complex of species. This has been resolved with the study of topotype populations of P. gutierrezi and P. panamaensis combined with rDNA sequences (D2-D3 expansion segments of 28S and ITS). Our results, including SEM en face view data on topotypes of P. gutierrezi and P. panamaensis demonstrated that both species are almost undistinguishable morphologically, but clearly separated molecularly with D2-D3 expansion segments of 28S (89 % similar, 84 nucleotides and 19 indels) and ITS rDNA (only 36 % coverage with a 88 % similarity value). Consequently, P. gutierrezi and P. panamaensis need to be maintained as different valid taxa. Molecular data on D2-D3 expansion segments of 28S and ITS rDNA of topotypes of P. panamaensis were also coincident (99 % similarity) with root-lesion nematodes parasitizing coffee in Guatemala and Costa Rica, and banana in Madeira, and previously identified as P. gutierrezi (K1-AF170440, K2-AF170441, FR692277, respectively), which need to be considered conspecific with P. panamaensis. These results showed us the necessity of integrative taxonomy in the identification and characterization of Pratylenchus spp.
format	Artículo
author	Zamora, Tatiana Peraza-Padilla, Walter Archidona Yuste, Antonio Cantalapiedra Navarrete, Carolina Liébanas Torres, Gracia María Palomares-Rius, Juan Emilio Castillo, Pablo
author_sort	Zamora, Tatiana
title	Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes
title_short	Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes
title_full	Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes
title_fullStr	Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes
title_full_unstemmed	Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes
title_sort	root-lesion nematodes of the genus pratylenchus (nematoda: pratylenchidae) from costa rica with molecular identification of p. gutierrezi and p. panamaensis topotypes
publisher	Springer
publishDate	2021
url	http://hdl.handle.net/11056/20595 https://doi.org/10.1007/s10658-016-0884-z
_version_	1796096590717386752
score	12.043282

Root-lesion nematodes of the genus Pratylenchus (Nematoda: Pratylenchidae) from Costa Rica with molecular identification of P. gutierrezi and P. panamaensis topotypes

Ejemplares similares