Identification of biological-active compounds in somatic callus cultures in Plantago major
With growing interest in the use of the properties of medicinal plants, the study of bioactive compounds thereof becomes a field of exciting new research, which seeks to relate and understand the synergy between natural substances and their positive impact on human health. An important utility of es...
| Autores Principales: | Jiménez-Quesada, Karol, Garro-Monge, Giovanni |
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| Formato: | Artículo |
| Idioma: | Español |
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Editorial Tecnológica de Costa Rica
2017
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https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3063 https://hdl.handle.net/2238/8693 |
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RepoTEC8693 |
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RepoTEC86932017-09-19T20:37:31Z Identification of biological-active compounds in somatic callus cultures in Plantago major Establecimiento de callogénesis somática en Plantago major e identificación de compuestos con actividad biológica Jiménez-Quesada, Karol Garro-Monge, Giovanni Plantago major; cell cultures; somatic embryogenesis; bioactive compounds; aucubin; verbascoside; iridoids Aucubina; compuestos bioactivos; cultivos celulares; embriogénesis somática; iridoides; Plantago major; verbascósidos With growing interest in the use of the properties of medicinal plants, the study of bioactive compounds thereof becomes a field of exciting new research, which seeks to relate and understand the synergy between natural substances and their positive impact on human health. An important utility of establishing cell cultures is precisely that they offer the opportunity to analyze the biochemical level industrial or clinical use having a given species due to the presence of certain compounds in their bodies with the ability to produce them at the cellular level and in vitro. In this work the induction of somatic callus formation from seed germination og Plantago major wasachieved in vitro, which were disinfected using a previously established protocol. Then leaf explants (1.0-1.5 cm2) of plants were taken vitro and cultured in semi-solid medium (M&S), for which a matrix of 28 treatments combining different concentrations of growth regulators (2,4-D and TDZ) in M&S medium. From this matrix 3 medium cultures (M14, M27 and M28) with the best results in the induction of callus formation as somatic embryogenesis were selected. For identification and quantification of bioactive compounds present in the extracts lyophilized callus analysis of two standards were performed to test their effectiveness as chemical markers in quantifying verbascosides and iridoids aucubin based on P. major. Hydro-alcoholic extracts of raw P. major, were prepared according to the protocols established by the company Laboratorios Lisan. They were prepared at a concentration of 10% w/v using 95% ethanol. With HPLC analysis for both markers signals with stable baseline and good resolution they were generated, being the most effective of verbascóside, so we continued with the evidence necessary to achieve establish a protocol of quantification in extracts from corns established. The established methodology for the detection and quantification of bioactive metabolites in this system can be refined based on an extraction solvent that gives stability to the extracts in a determined time period. Ante el creciente interés en el aprovechamiento de las propiedades de las plantas medicinales, el estudio de compuestos bioactivos de las mismas se convierte en un campo de investigación interesante y novedoso, con el cual se busca relacionar y comprender la sinergia entre las sustancias naturales y su impacto positivo sobre la salud humana. Una importante utilidad del establecimiento de cultivos celulares es justamente que éstos ofrecen la oportunidad de analizar a nivel bioquímico el aprovechamiento industrial o clínico que tiene una determinada especie debido a la presencia de ciertos compuestos en sus órganos, con la posibilidad de producirlos a nivel celular e in vitro. En este trabajo se logró la inducción de callogénesis somática partir de la germinación de semillas in vitro de Plantago major, las cuales fueron desinfectadas utilizando un protocolo previamente establecido. Luego se tomaron explantes de hoja (1.0-1,5 cm2) de las vitro plantas y fueron cultivados en medio semi–sólido (M&S), para lo cual se generó una matriz de 28 tratamientos que combinaban diferentes concentraciones de reguladores de crecimiento (2,4-D y TDZ) en medio M&S. A partir de esta matriz se seleccionaron 3 medios de cultivo (M14, M27 y M28) con los mejores resultados tanto en la inducción de callogénesis como para embriogénesis somática. Para la identificación y cuantificación de los compuestos bioactivos presentes en los extractos liofilizados de callos, se realizaron análisis de dos estándares para comprobar su efectividad como marcadores químicos en la cuantificación de verbascósidos e iridoides con base en aucubina en P. major. Los extractos hidro-alcohólicos crudos de P. major, fueron elaborados según los protocolos establecidos por la empresa Laboratorios Lisan. Los mismos se prepararon en una concentración de 10% p/v utilizando etanol al 95%. Con el análisis mediante HPLC para ambos marcadores se generaron señales con línea base estable y buena resolución, siendo más efectivo el de verbascósido, por lo que se continuó con las pruebas necesarias para lograr establecer un protocolo de cuantificación en extractos provenientes de los callos generados. La metodología establecida para la detección y cuantificación de los metabolitos bioactivos en este sistema de callogénesis somática, aún debe ser depurada con base en un solvente de extracción que otorgue estabilidad a los extractos en un período de tiempo determinado. 2017-04-21 2017-09-19T20:37:31Z 2017-09-19T20:37:31Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3063 10.18845/tm.v30i1.3063 https://hdl.handle.net/2238/8693 spa https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3063/pdf Copyright (c) 2017 Revista Tecnología en Marcha application/pdf Editorial Tecnológica de Costa Rica Revista Tecnología en Marcha; Vol. 30, Núm. 1: Enero-Marzo 2017; pág. 38-48 2215-3241 0379-3982 |
| institution |
Tecnológico de Costa Rica |
| collection |
Repositorio TEC |
| language |
Español |
| topic |
Plantago major; cell cultures; somatic embryogenesis; bioactive compounds; aucubin; verbascoside; iridoids Aucubina; compuestos bioactivos; cultivos celulares; embriogénesis somática; iridoides; Plantago major; verbascósidos |
| spellingShingle |
Plantago major; cell cultures; somatic embryogenesis; bioactive compounds; aucubin; verbascoside; iridoids Aucubina; compuestos bioactivos; cultivos celulares; embriogénesis somática; iridoides; Plantago major; verbascósidos Jiménez-Quesada, Karol Garro-Monge, Giovanni Identification of biological-active compounds in somatic callus cultures in Plantago major |
| description |
With growing interest in the use of the properties of medicinal plants, the study of bioactive compounds thereof becomes a field of exciting new research, which seeks to relate and understand the synergy between natural substances and their positive impact on human health. An important utility of establishing cell cultures is precisely that they offer the opportunity to analyze the biochemical level industrial or clinical use having a given species due to the presence of certain compounds in their bodies with the ability to produce them at the cellular level and in vitro. In this work the induction of somatic callus formation from seed germination og Plantago major wasachieved in vitro, which were disinfected using a previously established protocol. Then leaf explants (1.0-1.5 cm2) of plants were taken vitro and cultured in semi-solid medium (M&S), for which a matrix of 28 treatments combining different concentrations of growth regulators (2,4-D and TDZ) in M&S medium. From this matrix 3 medium cultures (M14, M27 and M28) with the best results in the induction of callus formation as somatic embryogenesis were selected. For identification and quantification of bioactive compounds present in the extracts lyophilized callus analysis of two standards were performed to test their effectiveness as chemical markers in quantifying verbascosides and iridoids aucubin based on P. major. Hydro-alcoholic extracts of raw P. major, were prepared according to the protocols established by the company Laboratorios Lisan. They were prepared at a concentration of 10% w/v using 95% ethanol. With HPLC analysis for both markers signals with stable baseline and good resolution they were generated, being the most effective of verbascóside, so we continued with the evidence necessary to achieve establish a protocol of quantification in extracts from corns established. The established methodology for the detection and quantification of bioactive metabolites in this system can be refined based on an extraction solvent that gives stability to the extracts in a determined time period. |
| format |
Artículo |
| author |
Jiménez-Quesada, Karol Garro-Monge, Giovanni |
| author_sort |
Jiménez-Quesada, Karol |
| title |
Identification of biological-active compounds in somatic callus cultures in Plantago major |
| title_short |
Identification of biological-active compounds in somatic callus cultures in Plantago major |
| title_full |
Identification of biological-active compounds in somatic callus cultures in Plantago major |
| title_fullStr |
Identification of biological-active compounds in somatic callus cultures in Plantago major |
| title_full_unstemmed |
Identification of biological-active compounds in somatic callus cultures in Plantago major |
| title_sort |
identification of biological-active compounds in somatic callus cultures in plantago major |
| publisher |
Editorial Tecnológica de Costa Rica |
| publishDate |
2017 |
| url |
https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3063 https://hdl.handle.net/2238/8693 |
| _version_ |
1796139257409044480 |
| score |
12.043409 |