Vista Equipo: Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation

Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation

The phenylalanine ammonium lyase protein or PAL is a key enzyme in the pathway of flavonoid synthesis; in strawberry 6 genes have been reported that encode it, including FaPAL2. Flavonoids are essential secondary metabolites for protection against UV light in plants, furthermore, they are of great p...

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Autores Principales:	Masís-Ramos, Stephannie, Garduño-Hernández, Jesús Alonso, Lozoy- Gloria, Edmundo, Garro-Monge, Giovanni
Formato:	Artículo
Idioma:	Español
Publicado:	Editorial Tecnológica de Costa Rica (entidad editora) 2021
Materias:	PAL flavonoids FaPAL2 UV-C light promoter TAIL PCR Fragaria ananassa GUS gene flavonoides luz UV-C promotor gen GUS
Acceso en línea:	https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/5207

id	RepoTEC13362
recordtype	dspace
spelling	RepoTEC133622022-04-23T03:54:20Z Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation Aislamiento de la región promotora del gen FaPAL2 de Fragaria x ananassa Cv. “Camino Real” y evaluación de su funcionalidad en respuesta a la irradiación UV-C Masís-Ramos, Stephannie Garduño-Hernández, Jesús Alonso Lozoy- Gloria, Edmundo Garro-Monge, Giovanni PAL flavonoids FaPAL2 UV-C light promoter TAIL PCR Fragaria ananassa GUS gene PAL flavonoides FaPAL2 luz UV-C promotor TAIL PCR Fragaria ananassa gen GUS The phenylalanine ammonium lyase protein or PAL is a key enzyme in the pathway of flavonoid synthesis; in strawberry 6 genes have been reported that encode it, including FaPAL2. Flavonoids are essential secondary metabolites for protection against UV light in plants, furthermore, they are of great pharmaceutical interest due to their antioxidants, antibacterial, anti-inflammatory, antimutagenic and anticancer properties. Increase in flavonoid compounds in strawberries irradiated with UV-C light has been correlated with high levels of FaPAL gene expression. In order to study and control the expression of genes of interest, it is indispensable to know the promoters functionality, so the present investigation aimed to identify and isolate the FaPal2 gene promoter using the TAIL PCR technique, to later assess its activity upon response to UV-C light in Fragaria x ananassa cv. “Camino Real” fruits via Agrobacterium tumefaciens using the GUS reporter gene. The promoter of the FaPAL2 gene was successfully isolated and sequenced, and later used to generate a genetic construct and evaluate its transient genetic expression in agro-infiltrated strawberry fruits. A positive histological staining was identified in the agro-infiltrated fruits, both irradiated and non-irradiated, indicating that the FaPAL2 gene promoter acts positively in response to UV-C light, but not in an exclusive manner. La proteína fenilalanina amonio-liasa o PAL es una enzima clave en la ruta de síntesis de los flavonoides; en fresa se han reportado 6 genes que la codifican, entre ellos el FaPAL2. Los flavonoides son metabolitos secundarios que participan en la protección contra luz UV de las plantas, además, son de gran interés farmacéutico debido a las propiedades antioxidantes, antibacterianas, antiinflamatorias, antimutagénicas y anticancerígenas que poseen. Se ha correlacionado el aumento de flavonoides en fresas irradiadas con luz UV-C con altos niveles de expresión del gen FaPAL. Para poder estudiar y controlar la expresión de genes de interés es indispensable conocer la funcionalidad de los promotores, por lo que la presente investigación se planteó por objetivo identificar y aislar el promotor del gen FaPAL2 mediante la técnica TAIL PCR, para posteriormente evaluar su actividad ante respuesta a la luz UV-C en frutos de Fragaria x ananassa cv. “Camino Real” vía Agrobacterium tumefaciens utilizando el gen reportero GUS. Se consiguió aislar y secuenciar el promotor del gen FaPAL2, para después generar un constructo genético y evaluar su expresión genética transitoria en frutos agroinfiltrados de fresa. Se identificó una tinción histológica positiva de los frutos agroinfiltrados, tanto irradiados como no irradiados, lo que indica que el promotor del gen FaPAL2 actúa positivamente en respuesta a luz UV-C, pero no de manera exclusiva. 2021-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/5207 10.18845/tm.v34i4.5207 spa https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/5207/5608 https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/5207/5625 Derechos de autor 2021 Revista Tecnología en Marcha https://creativecommons.org/licenses/by-nc-nd/4.0 application/pdf text/html Editorial Tecnológica de Costa Rica (entidad editora) Tecnología en marcha Journal; 2021: Vol. 34 Núm. 4: Octubre-Diciembre 2021; Pág. 105-117 Revista Tecnología en Marcha; 2021: Vol. 34 Núm. 4: Octubre-Diciembre 2021; Pág. 105-117 2215-3241 0379-3982
institution	Tecnológico de Costa Rica
collection	Repositorio TEC
language	Español
topic	PAL flavonoids FaPAL2 UV-C light promoter TAIL PCR Fragaria ananassa GUS gene PAL flavonoides FaPAL2 luz UV-C promotor TAIL PCR Fragaria ananassa gen GUS
spellingShingle	PAL flavonoids FaPAL2 UV-C light promoter TAIL PCR Fragaria ananassa GUS gene PAL flavonoides FaPAL2 luz UV-C promotor TAIL PCR Fragaria ananassa gen GUS Masís-Ramos, Stephannie Garduño-Hernández, Jesús Alonso Lozoy- Gloria, Edmundo Garro-Monge, Giovanni Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation
description	The phenylalanine ammonium lyase protein or PAL is a key enzyme in the pathway of flavonoid synthesis; in strawberry 6 genes have been reported that encode it, including FaPAL2. Flavonoids are essential secondary metabolites for protection against UV light in plants, furthermore, they are of great pharmaceutical interest due to their antioxidants, antibacterial, anti-inflammatory, antimutagenic and anticancer properties. Increase in flavonoid compounds in strawberries irradiated with UV-C light has been correlated with high levels of FaPAL gene expression. In order to study and control the expression of genes of interest, it is indispensable to know the promoters functionality, so the present investigation aimed to identify and isolate the FaPal2 gene promoter using the TAIL PCR technique, to later assess its activity upon response to UV-C light in Fragaria x ananassa cv. “Camino Real” fruits via Agrobacterium tumefaciens using the GUS reporter gene. The promoter of the FaPAL2 gene was successfully isolated and sequenced, and later used to generate a genetic construct and evaluate its transient genetic expression in agro-infiltrated strawberry fruits. A positive histological staining was identified in the agro-infiltrated fruits, both irradiated and non-irradiated, indicating that the FaPAL2 gene promoter acts positively in response to UV-C light, but not in an exclusive manner.
format	Artículo
author	Masís-Ramos, Stephannie Garduño-Hernández, Jesús Alonso Lozoy- Gloria, Edmundo Garro-Monge, Giovanni
author_sort	Masís-Ramos, Stephannie
title	Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation
title_short	Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation
title_full	Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation
title_fullStr	Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation
title_full_unstemmed	Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation
title_sort	isolation of the promoter region of the fapal2 gen of fragaria x ananassa cv. “camino real” and evaluation of it’s functionality in response to uv-c irradiation
publisher	Editorial Tecnológica de Costa Rica (entidad editora)
publishDate	2021
url	https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/5207
_version_	1796142033454235648
score	12.238652

Isolation of the promoter region of the FaPAL2 gen of Fragaria x ananassa Cv. “Camino Real” and evaluation of it’s functionality in response to UV-C irradiation

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