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Raman spectroscopy analysis of DNA using the SERS method

The technological advances of the last decades have contributed to the development of new methodologies for the characterization of biological samples. Raman Spectroscopy has been used for the characterization of pure inorganic and organic compounds, but recently, it is being used to study molecular...

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Autores Principales: Varela-Fonseca, Stephanie, Montero-Zeledón, Ernesto, Rojas- Rojas, Laura, Varela-Fonseca, Alejandro, Gutiérrez-Fallas, Dionisio
Formato: Artículo
Idioma: Español
Publicado: Editorial Tecnológica de Costa Rica (entidad editora) 2019
Materias:
Raman spectroscopy
SERS
nanoparticles
DNA
Espectroscopía Raman
nanopartículas
ADN
Acceso en línea: https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4501
https://hdl.handle.net/2238/11981
id RepoTEC11981
recordtype dspace
spelling RepoTEC119812020-09-25T23:12:38Z Raman spectroscopy analysis of DNA using the SERS method Análisis de ADN mediante espectroscopía Raman utilizando el método SERS Varela-Fonseca, Stephanie Montero-Zeledón, Ernesto Rojas- Rojas, Laura Varela-Fonseca, Alejandro Gutiérrez-Fallas, Dionisio Raman spectroscopy SERS nanoparticles DNA Espectroscopía Raman SERS nanopartículas ADN The technological advances of the last decades have contributed to the development of new methodologies for the characterization of biological samples. Raman Spectroscopy has been used for the characterization of pure inorganic and organic compounds, but recently, it is being used to study molecular structures of biological importance. Fluorescence is usually present in Raman spectroscopy, shielding Raman signals, but using the SERS (Surface Enhanced Raman Spectroscopy) technique, it is possible to locally increase the Raman signal and to reduce the importance of the fluorescence effects on the samples. The SERS effect could be achieved by adding metallic nanoparticles, of a specific size distribution, to the sample. Usually gold and silver nanoparticles are used for SERS. In this research, DNA of human and Rickettsia bacteria were characterized by the SERS technique, with both silver and gold nanoparticles. El avance tecnológico de las últimas décadas ha propiciado el desarrollo de nuevas metodologías para la caracterización de materiales de origen biológico. La espectroscopía Raman es utilizada principalmente en la caracterización de sustancias orgánicas e inorgánicas puras, sin embargo, recientemente se está utilizando para el estudio de organismos y estructuras moleculares de interés biológico. La principal dificultad en el análisis de estos materiales mediante la espectroscopía Raman es la fluorescencia que presentan las muestras al ser iluminadas con el haz de luz láser, pero mediante la técnica SERS (Surface Enhanced Raman Spectroscopy), es posible aumentar la señal Raman y reducir la interferencia de la fluorescencia. Para la aplicación de las técnicas SERS se pueden utilizar nanopartículas metálicas, principalmente oro y plata, de una adecuada distribución de tamaños. En este trabajo se presentan los resultados de la aplicación de técnica SERS a la caracterización espectroscópica del ADN de dos organismos diferentes, de un ser humano y de una bacteria del género Rickettsia, utilizando nanopartículas de oro y plata para la aplicación de la metodología. 2019-07-26 2020-09-25T23:12:38Z 2020-09-25T23:12:38Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4501 10.18845/tm.v32i3.4501 https://hdl.handle.net/2238/11981 spa https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4501/4081 https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4501/4265 application/pdf text/html Editorial Tecnológica de Costa Rica (entidad editora) Tecnología en marcha Journal; 2019: Vol. 32, Núm. 3: Julio-Setiembre 2019; Pág. 118-125 Revista Tecnología en Marcha; 2019: Vol. 32, Núm. 3: Julio-Setiembre 2019; Pág. 118-125 2215-3241 0379-3982
institution Tecnológico de Costa Rica
collection Repositorio TEC
language Español
topic Raman spectroscopy
SERS
nanoparticles
DNA
Espectroscopía Raman
SERS
nanopartículas
ADN
spellingShingle Raman spectroscopy
SERS
nanoparticles
DNA
Espectroscopía Raman
SERS
nanopartículas
ADN
Varela-Fonseca, Stephanie
Montero-Zeledón, Ernesto
Rojas- Rojas, Laura
Varela-Fonseca, Alejandro
Gutiérrez-Fallas, Dionisio
Raman spectroscopy analysis of DNA using the SERS method
description The technological advances of the last decades have contributed to the development of new methodologies for the characterization of biological samples. Raman Spectroscopy has been used for the characterization of pure inorganic and organic compounds, but recently, it is being used to study molecular structures of biological importance. Fluorescence is usually present in Raman spectroscopy, shielding Raman signals, but using the SERS (Surface Enhanced Raman Spectroscopy) technique, it is possible to locally increase the Raman signal and to reduce the importance of the fluorescence effects on the samples. The SERS effect could be achieved by adding metallic nanoparticles, of a specific size distribution, to the sample. Usually gold and silver nanoparticles are used for SERS. In this research, DNA of human and Rickettsia bacteria were characterized by the SERS technique, with both silver and gold nanoparticles.
format Artículo
author Varela-Fonseca, Stephanie
Montero-Zeledón, Ernesto
Rojas- Rojas, Laura
Varela-Fonseca, Alejandro
Gutiérrez-Fallas, Dionisio
author_sort Varela-Fonseca, Stephanie
title Raman spectroscopy analysis of DNA using the SERS method
title_short Raman spectroscopy analysis of DNA using the SERS method
title_full Raman spectroscopy analysis of DNA using the SERS method
title_fullStr Raman spectroscopy analysis of DNA using the SERS method
title_full_unstemmed Raman spectroscopy analysis of DNA using the SERS method
title_sort raman spectroscopy analysis of dna using the sers method
publisher Editorial Tecnológica de Costa Rica (entidad editora)
publishDate 2019
url https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4501
https://hdl.handle.net/2238/11981
_version_ 1796140952885133312
score 12.041648

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