Molecular identification of microorganisms isolated in handmade mining plants of Nicaragua
Bioprospecting is an investigation conducted to identify species, genes and products with current or potential uses by mankind. Therefore, this project was developed with the purpose of identifying potentially useful native strains for the engineering development of biotechnological processes, where...
| Autor Principal: | Páramo Aguilera, Leandro Alberto |
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| Formato: | Artículo |
| Idioma: | Español |
| Publicado: |
Universidad Tecnológica de Panamá
2021
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| Acceso en línea: |
https://revistas.utp.ac.pa/index.php/id-tecnologico/article/view/2921 https://ridda2.utp.ac.pa/handle/123456789/13115 |
| Sumario: |
Bioprospecting is an investigation conducted to identify species, genes and products with current or potential uses by mankind. Therefore, this project was developed with the purpose of identifying potentially useful native strains for the engineering development of biotechnological processes, where isolation techniques were applied, in addition to a recognition of the morphological characteristics of microorganisms by observing Gram stains, adhesive tapes and fresh stains; as well as the molecular identification by sequencing the 16S regions for bacteria and STIs and D1 / D2 / D3 for fungi. Concluded the work, a total of 66 microbial isolates were obtained, which were subjected to morphological tests and among these only 18 bacteria, 2 filamentous fungi, 3 yeast fungi were carried out molecular tests, which allowed to identify 6 different bacterial species and 5 fungal species, in addition to those identified up to genus level of Halomonas sp and Enterobacter sp. The fully identified strains belong to: bacteria such as (1) Acinetobacter calcoaceticus, (1) Aeromonas hydrophila, (4) Bacillus cereus, (1) Bacillus marisflavi, (1) Exiguobacterium aurantiacum and (1) Terribacillus saccharophilus and fungi such as: (1) Cyberlindnera jadinii, (1) Pichia jadinii, (1) Trichosporon asahii, (1) Penicillum janthinellum and (1) Penicillum simplicissimum. |
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