Phenotypic characterization and molecular identification of a luminescent marine bacterum isolated from the NW shelf of Cuba
Light emitted by luminescent bacteria is sensitive to several toxic compounds; therefore, some luminous species have been used to evaluate the quality of water environments. In the present study we carried out the phenotypic characterization and molecular identification of a marine luminescent...
Autores Principales: | Delgado Gómez, Yolaine, Umaña Castro, Rodolfo, Solano-González, Stefany, Iglesias Rodríguez, María Victoria, Ortiz Guilarte, Eudalys, Álvarez Valcárcel, Carlos, Lugioyo Gallardo, Gladys M. |
---|---|
Formato: | Artículo |
Idioma: | Inglés |
Publicado: |
Universidad de Sonora (México)
2024
|
Materias: | |
Acceso en línea: |
http://hdl.handle.net/11056/27710 https://doi.org/10.18633/biotecnia.v19i3.441 |
Sumario: |
Light emitted by luminescent bacteria is sensitive to
several toxic compounds; therefore, some luminous species
have been used to evaluate the quality of water environments.
In the present study we carried out the phenotypic
characterization and molecular identification of a marine
luminescent isolate (CBM-784) from the NW Cuban coast. The
identification of the CBM-784 luminous isolate was based on
phenotypic and genotypic characteristics. Phenotypically,
the CBM-784 strain revealed the following characteristics:
Gram negative, positive to oxidase and catalase reactions,
bioluminescent, and facultative anaerobic respiration. The
bacterial strain produces enzymes with gelatinase, lysine
carboxylase and amylase activity. Taken together, these
assays indicated that CBM-784 showed a high phenotypic
similarity to the Vibrio harveyi ATCC 14126 strain. On the basis
of 16S rRNA gene sequencing, CBM-784 was closely related
to Vibrio harveyi and Vibrio rotiferianus (94% similarity).
Sequence analysis of gyrB gene, has shown that CBM-784
shares taxonomic position with Vibrio campbellii and Vibrio
harveyi isolates, with 95% of bootstrap value. In addition,
sequence analysis of pyrH gene, grouped this isolate to the
Vibrio harveyi cluster with a strong bootstrap support (99%).
The multilocus sequence analysis and phenotypic characterization
of CBM-784 indicated that this strain have a strong
relation to Vibrio harveyi. |
---|