A bvrR/bvrS Non-Polar Brucella abortus Mutant Confirms the Role of the Two-Component System BvrR/BvrS in Virulence and Membrane Integrity
Brucella abortus is a bacterial pathogen causing bovine brucellosis worldwide. This facultative extracellular–intracellular pathogen can be transmitted to humans, leading to a zoonotic disease. The disease remains a public health concern, particularly in regions where livestock farming is present....
Autores Principales: | Rivas-Solano, Olga, Núñez-Montero, Kattia, Altamirano-Silva, Pamela, Ruiz-Villalobos, Nazareth, BARQUERO-CALVO, ELIAS, Moreno, Edgardo, Chaves-Olarte, Esteban, Guzman-Verri, Caterina |
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Formato: | Artículo |
Idioma: | Inglés |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: |
http://hdl.handle.net/11056/26175 https://doi.org/10.3390/microorganisms11082014 |
Sumario: |
Brucella abortus is a bacterial pathogen causing bovine brucellosis worldwide. This facultative extracellular–intracellular pathogen can be transmitted to humans, leading to a zoonotic disease.
The disease remains a public health concern, particularly in regions where livestock farming is
present. The two-component regulatory system BvrR/BvrS was described by isolating the attenuated
transposition mutants bvrR::Tn5 and bvrS::Tn5, whose characterization led to the understanding of
the role of the system in bacterial survival. However, a phenotypic comparison with deletion mutants
has not been performed because their construction has been unsuccessful in brucellae and difficult
in phylogenetically related Rhizobiales with BvrR/BvrS orthologs. Here, we used an unmarked
gene excision strategy to generate a B. abortus mutant strain lacking both genes, called B. abortus
∆bvrRS. The deletion was verified through PCR, Southern blot, Western blot, Sanger sequencing, and
whole-genome sequencing, confirming a clean mutation without further alterations at the genome
level. B. abortus ∆bvrRS shared attenuated phenotypic traits with both transposition mutants, confirming the role of BvrR/BvrS in pathogenesis and membrane integrity. This B. abortus ∆bvrRS with a
non-antimicrobial marker is an excellent tool for continuing studies on the role of BvrR/BvrS in the B.
abortus lifestyle. |
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