Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration
Artículo científico
| Autores Principales: | Chávez, Myra Noemi, Ludwig-Schenck, Thilo, Hopfner, Ursula, Centeno-Cerdas, Carolina, Somlai-Schweiger, Ian, Schwarz, Christian, Machens, Hans-Günther, Heikenwalder, Mathias, Bono, María Rosa, Allende, Miguel, Nickelsen, Jörg, Egaña, José Tomás |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
Biomaterials
2018
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| Acceso en línea: |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946422630&doi=10.1016%2fj.biomaterials.2015.10.014&partnerID=40&md5=0fdffd34fcee87539470fbe452fe7774 https://hdl.handle.net/2238/9849 |
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RepoTEC9849 |
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RepoTEC98492023-10-12T17:23:38Z Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration Chávez, Myra Noemi Ludwig-Schenck, Thilo Hopfner, Ursula Centeno-Cerdas, Carolina Somlai-Schweiger, Ian Schwarz, Christian Machens, Hans-Günther Heikenwalder, Mathias Bono, María Rosa Allende, Miguel Nickelsen, Jörg Egaña, José Tomás Biomateriales Algas Biotecnología Drogas Oxígeno Medicina Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics Biomaterials Algae Biotechnology Drugs Oxygen Medicine Artículo científico The use of artificial tissues in regenerative medicine is limited due to hypoxia. As a strategy to overcome this drawback, we have shown that photosynthetic biomaterials can produce and provide oxygen independently of blood perfusion by generating chimeric animal-plant tissues during dermal regeneration. In this work, we demonstrate the safety and efficacy of photosynthetic biomaterials in vivo after engraftment in a fully immunocompetent mouse skin defect model. Further, we show that it is also possible to genetically engineer such photosynthetic scaffolds to deliver other key molecules in addition to oxygen. As a proof-of-concept, biomaterials were loaded with gene modified microalgae expressing the angiogenic recombinant protein VEGF. Survival of the algae, growth factor delivery and regenerative potential were evaluated in vitro and in vivo. This work proposes the use of photosynthetic gene therapy in regenerative medicine and provides scientific evidence for the use of engineered microalgae as an alternative to deliver recombinant molecules for gene therapy. 2018-08-03T20:02:49Z 2018-08-03T20:02:49Z 2016 info:eu-repo/semantics/article https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946422630&doi=10.1016%2fj.biomaterials.2015.10.014&partnerID=40&md5=0fdffd34fcee87539470fbe452fe7774 Chávez, M., Ludwig-Schenck, T., Hopfner, U., Centeno-Cerdas, C., Somlai-Schweiger, I., Schwarz, C., Machens, H., Heikenwalder, M., Bono, M., Allende, M., Nickelsen, J., & Egaña, J. (2016). Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration. Biomaterials, 75, 25-36. 1429612 https://hdl.handle.net/2238/9849 eng 10.1016/j.biomaterials.2015.10.014 application/pdf Biomaterials Biomaterials |
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Tecnológico de Costa Rica |
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Repositorio TEC |
| language |
Inglés |
| topic |
Biomateriales Algas Biotecnología Drogas Oxígeno Medicina Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics Biomaterials Algae Biotechnology Drugs Oxygen Medicine |
| spellingShingle |
Biomateriales Algas Biotecnología Drogas Oxígeno Medicina Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Genetics Biomaterials Algae Biotechnology Drugs Oxygen Medicine Chávez, Myra Noemi Ludwig-Schenck, Thilo Hopfner, Ursula Centeno-Cerdas, Carolina Somlai-Schweiger, Ian Schwarz, Christian Machens, Hans-Günther Heikenwalder, Mathias Bono, María Rosa Allende, Miguel Nickelsen, Jörg Egaña, José Tomás Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration |
| description |
Artículo científico |
| format |
Artículo |
| author |
Chávez, Myra Noemi Ludwig-Schenck, Thilo Hopfner, Ursula Centeno-Cerdas, Carolina Somlai-Schweiger, Ian Schwarz, Christian Machens, Hans-Günther Heikenwalder, Mathias Bono, María Rosa Allende, Miguel Nickelsen, Jörg Egaña, José Tomás |
| author_sort |
Chávez, Myra Noemi |
| title |
Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration |
| title_short |
Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration |
| title_full |
Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration |
| title_fullStr |
Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration |
| title_full_unstemmed |
Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration |
| title_sort |
towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration |
| publisher |
Biomaterials |
| publishDate |
2018 |
| url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946422630&doi=10.1016%2fj.biomaterials.2015.10.014&partnerID=40&md5=0fdffd34fcee87539470fbe452fe7774 https://hdl.handle.net/2238/9849 |
| _version_ |
1796143903524519936 |
| score |
12.043282 |