Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis
Artículo científico
| Autores Principales: | Schmidt-Durán, Alexander, Alvarado-Ulloa, Carlos, Chacón-Cerdas, Randall, Alvarado-Marchena, Luis, Flores-Mora, Dora María |
|---|---|
| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
Springer
2018
|
| Materias: | |
| Acceso en línea: |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052243/ https://hdl.handle.net/2238/9644 |
| id |
RepoTEC9644 |
|---|---|
| recordtype |
dspace |
| spelling |
RepoTEC96442023-10-12T21:31:09Z Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis Schmidt-Durán, Alexander Alvarado-Ulloa, Carlos Chacón-Cerdas, Randall Alvarado-Marchena, Luis Flores-Mora, Dora María Células -- Salud Antioxidantes Polifenoles Frutas Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Cell biology Ácidos fenólicos Mora tropical Cells -- Health Antioxidants Polyphenols Fruits Phenolic acids Tropical blackberry Artículo científico Blackberries are fruits produced worldwide, with 25 % of their production centered in Mexico, Central and South America. Tropical highland blackberry is a fruit that can potentially enhance human health, due to their high content in phenolic compounds, which include anthocyanins, phenolic acids, tannins (gallotannins and elagitannins) and flavonoids. Therefore, the overall aim of this study is the development of a callus induction protocol, the establishment of blackberry cell suspensions (Rubus adenotrichos Schltdl.) and their cell analysis through optical microscopy and TEM, for the potential production of phenolic compounds. In order to produce callogenesis, segments of blackberry leaves were disinfected and placed in different concentrations of 2,4-D and the control media (0; 0.5; 1.0; 1.5; 2.0; 2.5 and 3.0 mg/l of 2,4-D); obtaining the higher size of calli in the medium with 1.5 mg/l of 2,4-D. After this determination, and for this specific treatment, a growth curve was performed through the use of fresh and dry weight parameters, in order to identify each of the growth stages. Furthermore, the calli obtained from the 1.5 mg/l of 2,4-D treatment were placed in two different culture media (MS and MS supplemented with 1.5 mg/l of 2,4-D) in order to establish the cell suspensions and the growth curve. To the best treatment, the total polyphenols were also quantified. It was determined that the MS medium is ideal for the growth and disintegration of the cell suspensions, obtaining 0.0256 mg of gallic acid/g of fresh sample. Finally, a cell callus and cell suspension analysis was performed through OM and TEM, evidencing a higher hystological differentiation in the calli, as well as the observation of antioxidant storage in the plastids. 2018-03-07T20:39:54Z 2018-03-07T20:39:54Z 2016 info:eu-repo/semantics/article https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052243/ Schmidt-Durán, A., Alvarado-Ulloa, C., Chacón-Cerdas, R., Alvarado-Marchena, L., & Flores-Mora, D. (2016). Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis. SpringerPlus, 5(1). 21931801 https://hdl.handle.net/2238/9644 eng 10.1186/s40064-016-3381-0 Attribution-NonCommercial-ShareAlike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Springer SpringerPlus |
| institution |
Tecnológico de Costa Rica |
| collection |
Repositorio TEC |
| language |
Inglés |
| topic |
Células -- Salud Antioxidantes Polifenoles Frutas Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Cell biology Ácidos fenólicos Mora tropical Cells -- Health Antioxidants Polyphenols Fruits Phenolic acids Tropical blackberry |
| spellingShingle |
Células -- Salud Antioxidantes Polifenoles Frutas Research Subject Categories::NATURAL SCIENCES::Biology::Cell and molecular biology::Cell biology Ácidos fenólicos Mora tropical Cells -- Health Antioxidants Polyphenols Fruits Phenolic acids Tropical blackberry Schmidt-Durán, Alexander Alvarado-Ulloa, Carlos Chacón-Cerdas, Randall Alvarado-Marchena, Luis Flores-Mora, Dora María Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis |
| description |
Artículo científico |
| format |
Artículo |
| author |
Schmidt-Durán, Alexander Alvarado-Ulloa, Carlos Chacón-Cerdas, Randall Alvarado-Marchena, Luis Flores-Mora, Dora María |
| author_sort |
Schmidt-Durán, Alexander |
| title |
Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis |
| title_short |
Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis |
| title_full |
Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis |
| title_fullStr |
Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis |
| title_full_unstemmed |
Callogenesis and cell suspension establishment of tropical highland blackberry (Rubus adenotrichos Schltdl.) and its microscopic analysis |
| title_sort |
callogenesis and cell suspension establishment of tropical highland blackberry (rubus adenotrichos schltdl.) and its microscopic analysis |
| publisher |
Springer |
| publishDate |
2018 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052243/ https://hdl.handle.net/2238/9644 |
| _version_ |
1796140927467651072 |
| score |
12.043418 |