Molecular characterization and genetic diversity of Jatropha curcas L. in Costa Rica
We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS markers. Non-toxicity was evaluated using G-SSRs and S...
Autores Principales: | Vásquez-Mayorga, Marcela, Fuchs, Eric, Hernández, Eduardo, Herrera, Franklin, Hernández, Jesús, Moreira-González, Ileana, Arnáez-Serrano, Elizabeth, Barboza, Natalia |
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Formato: | Artículo |
Idioma: | Español |
Publicado: |
2017
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Materias: | |
Acceso en línea: |
https://peerj.com/articles/2931/ https://hdl.handle.net/2238/7095 |
Sumario: |
We estimated the genetic diversity of 50 Jatropha curcas samples from the Costa Rican
germplasm bank using 18 EST-SSR, one G-SSR and nrDNA-ITS markers. We also
evaluated the phylogenetic relationships among samples using nuclear ribosomal ITS
markers. Non-toxicity was evaluated using G-SSRs and SCARs markers. A Neighbor-
Joining (NJ) tree and a Maximum Likelihood (ML) tree were constructed using SSR
markers and ITS sequences, respectively. Heterozygosity was moderate (He D 0.346),
but considerable compared to worldwide values for J. curcas. The PIC (PIC D 0.274)
and inbreeding coefficient (f D0:102) were both low. Clustering was not related to
the geographical origin of accessions. International accessions clustered independently
of collection sites, suggesting a lack of genetic structure, probably due to the wide
distribution of this crop and ample gene flow. Molecular markers identified only one
non-toxic accession (JCCR-24) from Mexico. This work is part of a countrywide effort
to characterize the genetic diversity of the Jatropha curcas germplasm bank in Costa
Rica. |
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