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Evaluation of skin procurement and storage techniques for Tissue Banking

Here we report how we reproduced and analyzed the most common international techniques for managing skin for Tissue Banking. Skin ablation, processing, disinfection and storage were performed using an animal model (domestic pig, Sus scrofa domesticus). Two different disinfection methods were compare...

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Autores Principales: Calvo-Castro, Laura A., Guerrero-Barrantes, Maritza, Ulloa-Fernández, Andrea, Portuguez-Barboza, Rafael, Centeno-Cerdas, Carolina, Rojas-Chaves, Miguel
Formato: Artículo
Idioma: Español
Publicado: Editorial Tecnológica de Costa Rica 2015
Materias:
Electron beam irradiation; Dakins solution; glycerol; cryopreservation.
Irradiación con haz de electrones; solución de Dakins; glicerol; criopreservación.
Acceso en línea: https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/2221
https://hdl.handle.net/2238/5920
id RepoTEC5920
recordtype dspace
spelling RepoTEC59202018-09-04T19:57:09Z Evaluation of skin procurement and storage techniques for Tissue Banking Evaluación de técnicas de procesamiento y almacenamiento de piel cadavérica para bancos de tejidos Calvo-Castro, Laura A. Guerrero-Barrantes, Maritza Ulloa-Fernández, Andrea Portuguez-Barboza, Rafael Centeno-Cerdas, Carolina Rojas-Chaves, Miguel Electron beam irradiation; Dakins solution; glycerol; cryopreservation. Irradiación con haz de electrones; solución de Dakins; glicerol; criopreservación. Here we report how we reproduced and analyzed the most common international techniques for managing skin for Tissue Banking. Skin ablation, processing, disinfection and storage were performed using an animal model (domestic pig, Sus scrofa domesticus). Two different disinfection methods were compared (antibiotics and chemical disinfection with chlorine) by evaluating the microbiology profile of the tissue. Also, two different storage and preservation treatments were compared: glycerol immersion at low temperatures (4°C) and cryopreservation (-70°C). Finally, we analyzed the sterilization of the tissue by electron beam irradiation (25 kGy). General structural changes in the tissue were analyzed by histological staining. Our results show that complete sterilization of the samples was achieved in both the irradiated tissue and in the chemically disinfected glycerolated skin (Dakins solution). Histological comparison between treatments revealed that samples kept in glycerol showed the best structural quality. En el presente trabajo se reproducen y analizan algunas de las técnicas más comunes a nivel internacional para el manejo de piel cadavérica para bancos de tejidos. Para ello, se llevó a cabo la ablación, el procesamiento, la desinfección y el almacenamiento de piel utilizando un modelo animal (cerdo, Sus scrofa domesticus). Para verificar la calidad microbiológica del tejido se compararon dos diferentes métodos de desinfección (antibióticos y desinfección química con cloro). Además, se evaluó el efecto de dos tratamientos para el almacenamiento y conservación de la piel: inmersión en glicerol a bajas temperaturas (4 °C) y criopreservación (-70 °C). Finalmente, se analizó un método de esterilización utilizando irradiación con haz de electrones (25 kGy). La estructura general del tejido se analizó mediante tinciones histológicas. Los resultados demostraron la esterilización completa de las muestras, tanto de los tejidos irradiados como en la piel glicerolada desinfectada químicamente (solución de Dakins). Por otro lado, la comparación histológica de las muestras reveló que el tejido glicerolado fue el que mostró la mejor calidad en términos estructurales.  2015-05-14 2015-05-22T16:03:19Z 2015-05-22T16:03:19Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/2221 https://hdl.handle.net/2238/5920 spa https://tecdigital.tec.ac.cr/servicios/ojs/index.php/tec_marcha/article/view/2221/2016 Copyright (c) 2015 Tecnología en Marcha application/pdf Editorial Tecnológica de Costa Rica Tecnología en Marcha; 2015: Revista Tecnología en Marcha. Ingeniería de Tejidos. Mayo 2015; pág. 69-82 2215-3241 0379-3982
institution Tecnológico de Costa Rica
collection Repositorio TEC
language Español
topic Electron beam irradiation; Dakins solution; glycerol; cryopreservation.
Irradiación con haz de electrones; solución de Dakins; glicerol; criopreservación.
spellingShingle Electron beam irradiation; Dakins solution; glycerol; cryopreservation.
Irradiación con haz de electrones; solución de Dakins; glicerol; criopreservación.
Calvo-Castro, Laura A.
Guerrero-Barrantes, Maritza
Ulloa-Fernández, Andrea
Portuguez-Barboza, Rafael
Centeno-Cerdas, Carolina
Rojas-Chaves, Miguel
Evaluation of skin procurement and storage techniques for Tissue Banking
description Here we report how we reproduced and analyzed the most common international techniques for managing skin for Tissue Banking. Skin ablation, processing, disinfection and storage were performed using an animal model (domestic pig, Sus scrofa domesticus). Two different disinfection methods were compared (antibiotics and chemical disinfection with chlorine) by evaluating the microbiology profile of the tissue. Also, two different storage and preservation treatments were compared: glycerol immersion at low temperatures (4°C) and cryopreservation (-70°C). Finally, we analyzed the sterilization of the tissue by electron beam irradiation (25 kGy). General structural changes in the tissue were analyzed by histological staining. Our results show that complete sterilization of the samples was achieved in both the irradiated tissue and in the chemically disinfected glycerolated skin (Dakins solution). Histological comparison between treatments revealed that samples kept in glycerol showed the best structural quality.
format Artículo
author Calvo-Castro, Laura A.
Guerrero-Barrantes, Maritza
Ulloa-Fernández, Andrea
Portuguez-Barboza, Rafael
Centeno-Cerdas, Carolina
Rojas-Chaves, Miguel
author_sort Calvo-Castro, Laura A.
title Evaluation of skin procurement and storage techniques for Tissue Banking
title_short Evaluation of skin procurement and storage techniques for Tissue Banking
title_full Evaluation of skin procurement and storage techniques for Tissue Banking
title_fullStr Evaluation of skin procurement and storage techniques for Tissue Banking
title_full_unstemmed Evaluation of skin procurement and storage techniques for Tissue Banking
title_sort evaluation of skin procurement and storage techniques for tissue banking
publisher Editorial Tecnológica de Costa Rica
publishDate 2015
url https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/2221
https://hdl.handle.net/2238/5920
_version_ 1796140720307830784
score 12.041648

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