Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples
Adipose-derived stem cells have great potential for tissue repair because of their multidifferentiation potential, growth factors and cytokines secreted. Currently, there is a vast portion of preclinical and clinical investigations aimed to treat skin wounds, spinal cord injuries and ischemia in the...
| Autores Principales: | Castro-Piedra, Silvia, Morales-Sánchez, Johan |
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| Formato: | Artículo |
| Idioma: | Inglés |
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Editorial Tecnológica de Costa Rica (entidad editora)
2019
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| Acceso en línea: |
https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4793 https://hdl.handle.net/2238/12021 |
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RepoTEC12021 |
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RepoTEC120212020-09-25T23:12:43Z Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples Aislamiento de células madre mesenquimales derivadas de tejido adiposo del pliegue inguinal murino: un protocolo para obtener lo mejor de pequeñas muestras Castro-Piedra, Silvia Morales-Sánchez, Johan Célula madre ASC regeneración andamio diferenciación Mesenchymal stem cell adipose-derived stem cells regeneration scaffold differentiation Adipose-derived stem cells have great potential for tissue repair because of their multidifferentiation potential, growth factors and cytokines secreted. Currently, there is a vast portion of preclinical and clinical investigations aimed to treat skin wounds, spinal cord injuries and ischemia in the myocardium with promising results. In this study, a small sample of adipose tissue was extracted from mice and the stromal vascular fraction was isolated. Then, it was cultivated in vitro in order to analyze the growth kinetics, expression of stem cell markers and differentiation potential. Stem cells cultivated and expanded from the stromal vascular fraction, showed similar kinetics to that reported in literature. A proportion of analyzed cells showed positivity for the stem cell markers and they were able to differentiate efficiently in adipogenic, chondrogenic and osteogenic lineages. The protocol established in this work allowed to isolate adipose-derived stem cells from a very small sample that met almost all the criteria established by the International Society for Cell and Gene Therapy, giving rise to future research for stablish this tissue as an excellent source of stem cells and the development of alternative therapies to the treatment of wounds. Las células madre se caracterizan por autorrenovación y diferenciación en varios tipos de células. Las primeras células madre identificadas fueron las hematopoyéticas; sin embargo, existen diferentes tipos, de diferente origen y potencial de diferenciación, por ejemplo, las células madres adultas mesenquimales, aisladas de médula ósea a mediados del siglo pasado. Actualmente se sabe que son población quiescente de muchos tejidos adultos, como el tejido adiposo. Estas células cuentan con gran potencial de diferenciación in vitro, son fuente de factores de crecimiento y citoquinas que participan en la reparación de tejidos. Por lo anterior, son protagonistas investigaciones preclínicas y clínicas para tratar heridas en piel, lesiones de médula espinal, isquemia en el miocardio, con resultados prometedores. Para este estudio, se extrajo tejido adiposo de ratones y se aisló la fracción vascular estromal. Esta, se cultivó in vitro para que las células que tuvieran adherencia al plástico crecieran. Posteriormente se les analizó la cinética de crecimiento, expresión de los marcadores de células madre y potencial de diferenciación. Las células lograron crecer y proliferar in vitro y mostraron una cinética similar a la reportada para estas células. Un veinticinco por ciento de las células cultivadas, mostraron positividad para los marcadores de células madre y se lograron diferenciar eficientemente en linajes adipogénico, condrogénico y osteogénico. El protocolo establecido en el presente trabajo permitió cumplir con la mayor parte de los criterios establecidos por la Sociedad Internacional para Terapia Celular y Génica, dando pie a futuras investigaciones para el desarrollo de terapias alternativas al tratamiento de heridas. 2019-10-23 2020-09-25T23:12:43Z 2020-09-25T23:12:43Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4793 10.18845/tm.v32i4.4793 https://hdl.handle.net/2238/12021 eng https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4793/4563 application/pdf Editorial Tecnológica de Costa Rica (entidad editora) Tecnología en marcha Journal; 2019: Vol. 32 Núm. 4 Octubre-Diciembre; Pág. 69-80 Revista Tecnología en Marcha; 2019: Vol. 32 Núm. 4 Octubre-Diciembre; Pág. 69-80 2215-3241 0379-3982 |
| institution |
Tecnológico de Costa Rica |
| collection |
Repositorio TEC |
| language |
Inglés |
| topic |
Célula madre ASC regeneración andamio diferenciación Mesenchymal stem cell adipose-derived stem cells regeneration scaffold differentiation |
| spellingShingle |
Célula madre ASC regeneración andamio diferenciación Mesenchymal stem cell adipose-derived stem cells regeneration scaffold differentiation Castro-Piedra, Silvia Morales-Sánchez, Johan Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples |
| description |
Adipose-derived stem cells have great potential for tissue repair because of their multidifferentiation potential, growth factors and cytokines secreted. Currently, there is a vast portion of preclinical and clinical investigations aimed to treat skin wounds, spinal cord injuries and ischemia in the myocardium with promising results. In this study, a small sample of adipose tissue was extracted from mice and the stromal vascular fraction was isolated. Then, it was cultivated in vitro in order to analyze the growth kinetics, expression of stem cell markers and differentiation potential. Stem cells cultivated and expanded from the stromal vascular fraction, showed similar kinetics to that reported in literature. A proportion of analyzed cells showed positivity for the stem cell markers and they were able to differentiate efficiently in adipogenic, chondrogenic and osteogenic lineages. The protocol established in this work allowed to isolate adipose-derived stem cells from a very small sample that met almost all the criteria established by the International Society for Cell and Gene Therapy, giving rise to future research for stablish this tissue as an excellent source of stem cells and the development of alternative therapies to the treatment of wounds. |
| format |
Artículo |
| author |
Castro-Piedra, Silvia Morales-Sánchez, Johan |
| author_sort |
Castro-Piedra, Silvia |
| title |
Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples |
| title_short |
Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples |
| title_full |
Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples |
| title_fullStr |
Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples |
| title_full_unstemmed |
Mesenchymal Stem Cell Isolation from Mice Inguinal Fat Tissue: a protocol to get the best from small samples |
| title_sort |
mesenchymal stem cell isolation from mice inguinal fat tissue: a protocol to get the best from small samples |
| publisher |
Editorial Tecnológica de Costa Rica (entidad editora) |
| publishDate |
2019 |
| url |
https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/4793 https://hdl.handle.net/2238/12021 |
| _version_ |
1796140686964162560 |
| score |
12.043409 |