First report of a Multidrug-Resistant ST58 Escherichia coli Harboring Extended-Spectrum Beta-Lactamase of the CTX-M-1 Class in a Fecal Sample of a Captive Baird’s Tapir (Tapirus bairdii) in Costa Rica, Central America
Objective: This study’s main objective was to analyze the antibiotic susceptibility profile of Escherichia coli isolates obtained from a fecal sample of a captive Baird’s tapir (Tapirus bairdii) in Costa Rica. Materials and Methods: The fecal sample was collected inside the enclosure on March 3, 2...
| Autores Principales: | Rojas Jiménez, Jorge, Jiménez-Pearson, María Antonieta, Duarte-Martínez, Francisco, Brenes Mora, Esteban, Arguedas, Randall, BARQUERO-CALVO, ELIAS |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
Mary Ann Liebert
2022
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| Materias: | |
| Acceso en línea: |
https://www.researchgate.net/publication/353498321 http://hdl.handle.net/11056/24359 |
| Sumario: |
Objective: This study’s main objective was to analyze the antibiotic susceptibility profile of Escherichia coli
isolates obtained from a fecal sample of a captive Baird’s tapir (Tapirus bairdii) in Costa Rica.
Materials and Methods: The fecal sample was collected inside the enclosure on March 3, 2017, right after the
animal defecated. Samples were cultured on MacConkey agar plates nonsupplemented and supplemented with
2 mg/mL of cefotaxime. Bacterial identification and antibiotic susceptibility were performed with the Vitek 2
Compact System and the Kirby Bauer disk diffusion method, respectively. Polymerase chain reaction ampli-
fication was performed to detect blaCTX-M beta-lactamase genes. Resistant isolates were subjected to whole-
genome sequencing (WGS).
Results: After evaluating several antibiotic classes, a multidrug-resistant E. coli strain with extended-spectrum
beta-lactamase phenotype was isolated. Resistance to cefotaxime, cefepime, ampicillin, ampicillin/sulbactam,
and tetracycline was detected. WGS analysis showed the presence of blaCTX-M-1, blaTEM-1B, and tet(B) genes.
The presence of IncN plasmids and Col156 was also detected.
Conclusion: Our findings are according with the notion that animals’ high density enhances the spread of
resistant determinants in a captive environment in a limited space, where the likelihood of direct or indirect
contact with other animals and humans is more frequent. |
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