Regulation of Brucella virulence by the two-component system BvrR/BvrS

The Brucella BvrR/BvrS two-component regulatory system is highly similar to the regulatory and sensory proteins of Sinorhizobium and Agrobacterium necessary for endosymbiosis and pathogenicity in plants, and very similar to a putative system present in the animal pathogen Bartonella. Mutations in th...

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Main Authors: López-Goñi, Ignacio, Guzman-Verri, Caterina, Manterola, Lorea, Sola-Landa, Alberto, Moriyon, Ignacio, Moreno, Edgardo
Format: Artículo
Language: Inglés
Published: Elsevier B.V. 2020
Subjects:
Online Access: http://hdl.handle.net/11056/18428
https://doi.org/10.1016/S0378-1135(02)00218-3
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spelling RepoUNACR184282022-04-07T21:14:34Z Regulation of Brucella virulence by the two-component system BvrR/BvrS López-Goñi, Ignacio Guzman-Verri, Caterina Manterola, Lorea Sola-Landa, Alberto Moriyon, Ignacio Moreno, Edgardo BvrR/BvrS BRUCELOSIS BRUCELLA VIRULENCE PROTEÍNAS MEMBRANAS CELULARES The Brucella BvrR/BvrS two-component regulatory system is highly similar to the regulatory and sensory proteins of Sinorhizobium and Agrobacterium necessary for endosymbiosis and pathogenicity in plants, and very similar to a putative system present in the animal pathogen Bartonella. Mutations in the bvrR or bvrS genes hamper the penetration of B. abortus in non-phagocytic cells and impairs intracellular trafficking and virulence. In contrast to virulent Brucella, BvrR/BvrS mutants do not recruit small GTPases of the Rho subfamily required for actin polymerization and penetration to cells. Dysfunction of the BvrR/BvrS system alters the outer membrane permeability, the expression of several group 3 outer membrane proteins and the pattern of lipid A acylation. Constructs of virulent B. abortus chimeras containing heterologous LPS from the bvrS− mutant demonstrated an altered permeability to cationic peptides similar to that of the BvrR/BvrS mutants. We hypothesize that the Brucella BvrR/BvrS is a system devoted to the homeostasis of the outer membrane and, therefore in the interface for cell invasion and mounting the required structures for intracellular parasitism. l sistema regulador de dos componentes Brucella BvrR / BvrS es muy similar a las proteínas reguladoras y sensoriales de Sinorhizobium y Agrobacterium necesarias para la endosimbiosis y patogenicidad en plantas, y muy similar a un sistema putativo presente en el patógeno animal Bartonella . Las mutaciones en los genes bvrR o bvrS dificultan la penetración de B. abortus en células no fagocíticas y altera el tráfico intracelular y la virulencia. En contraste con la virulenta Brucella, Los mutantes BvrR / BvrS no reclutan pequeñas GTPasas de la subfamilia Rho requeridas para la polimerización de actina y la penetración en las células. La disfunción del sistema BvrR / BvrS altera la permeabilidad de la membrana externa, la expresión de varias proteínas de la membrana externa del grupo 3 y el patrón de acilación del lípido A. Las construcciones de virulenta B. abortus quimeras que contienen LPS heterólogos a partir de los BVRS - mutantes demostraron una permeabilidad alterada a péptidos catiónicos similares a la de los / BVRS mutantes BvrR. Nuestra hipótesis es que Brucella BvrR / BvrS es un sistema dedicado a la homeostasis de la membrana externa y, por lo tanto, en la interfaz para la invasión celular y el montaje de las estructuras necesarias para el parasitismo intracelular. Universidad Nacional, Costa Rica Escuela de Medicina Veterinaria 2020-10-23T20:50:43Z 2020-10-23T20:50:43Z 2002-12-20 http://purl.org/coar/resource_type/c_6501 http://hdl.handle.net/11056/18428 https://doi.org/10.1016/S0378-1135(02)00218-3 eng Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier B.V. Veterinary Microbiology Vol. 90, No. 1–4, 20 Dec. 2002, Pag 329-339
institution Universidad Nacional de Costa Rica
collection Repositorio UNA-Costa Rica
language Inglés
topic BvrR/BvrS
BRUCELOSIS
BRUCELLA
VIRULENCE
PROTEÍNAS
MEMBRANAS CELULARES
spellingShingle BvrR/BvrS
BRUCELOSIS
BRUCELLA
VIRULENCE
PROTEÍNAS
MEMBRANAS CELULARES
López-Goñi, Ignacio
Guzman-Verri, Caterina
Manterola, Lorea
Sola-Landa, Alberto
Moriyon, Ignacio
Moreno, Edgardo
Regulation of Brucella virulence by the two-component system BvrR/BvrS
description The Brucella BvrR/BvrS two-component regulatory system is highly similar to the regulatory and sensory proteins of Sinorhizobium and Agrobacterium necessary for endosymbiosis and pathogenicity in plants, and very similar to a putative system present in the animal pathogen Bartonella. Mutations in the bvrR or bvrS genes hamper the penetration of B. abortus in non-phagocytic cells and impairs intracellular trafficking and virulence. In contrast to virulent Brucella, BvrR/BvrS mutants do not recruit small GTPases of the Rho subfamily required for actin polymerization and penetration to cells. Dysfunction of the BvrR/BvrS system alters the outer membrane permeability, the expression of several group 3 outer membrane proteins and the pattern of lipid A acylation. Constructs of virulent B. abortus chimeras containing heterologous LPS from the bvrS− mutant demonstrated an altered permeability to cationic peptides similar to that of the BvrR/BvrS mutants. We hypothesize that the Brucella BvrR/BvrS is a system devoted to the homeostasis of the outer membrane and, therefore in the interface for cell invasion and mounting the required structures for intracellular parasitism.
format Artículo
author López-Goñi, Ignacio
Guzman-Verri, Caterina
Manterola, Lorea
Sola-Landa, Alberto
Moriyon, Ignacio
Moreno, Edgardo
author_sort López-Goñi, Ignacio
title Regulation of Brucella virulence by the two-component system BvrR/BvrS
title_short Regulation of Brucella virulence by the two-component system BvrR/BvrS
title_full Regulation of Brucella virulence by the two-component system BvrR/BvrS
title_fullStr Regulation of Brucella virulence by the two-component system BvrR/BvrS
title_full_unstemmed Regulation of Brucella virulence by the two-component system BvrR/BvrS
title_sort regulation of brucella virulence by the two-component system bvrr/bvrs
publisher Elsevier B.V.
publishDate 2020
url http://hdl.handle.net/11056/18428
https://doi.org/10.1016/S0378-1135(02)00218-3
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score 12.248452