Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease

Brucellosis, caused by Brucella abortus is a major disease of cattle and a zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), a total 765 herds (13078 bovines) from six regions of CR were randomly sampled during 2012–2013. A non-random sample of 7907 herds (53219...

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Main Authors: Hernández-Mora, Gabriela, Ruiz-Villalobos, Nazareth, Bonilla-Montoya, Roberto, Romero-Zúñiga, Juan José, Jiménez-Arias, Julio, González-Barrientos, Rocio, BARQUERO-CALVO, ELIAS, Chacón-Díaz, Carlos, Rojas, Norman, Chaves-Olarte, Esteban, Guzman-Verri, Caterina, Moreno, Edgardo
Format: Artículo
Language: Inglés
Published: PLOS ONE 2020
Subjects:
Online Access: http://hdl.handle.net/11056/18345
https://doi.org/10.1371/journal.pone.0182380
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spelling RepoUNACR183452020-10-14T09:02:06Z Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease Hernández-Mora, Gabriela Ruiz-Villalobos, Nazareth Bonilla-Montoya, Roberto Romero-Zúñiga, Juan José Jiménez-Arias, Julio González-Barrientos, Rocio BARQUERO-CALVO, ELIAS Chacón-Díaz, Carlos Rojas, Norman Chaves-Olarte, Esteban Guzman-Verri, Caterina Moreno, Edgardo COSTA RICA BRUCELOSIS BRUCELLA EPIDEMIOLOGÍA BOVINOS ENFERMEDADES EN BOVINOS EPIDEMIOLOGIA VETERINARIA EPIDEMIOLOGY CATTLE DISEASES IN BOVINES VETERINARY EPIDEMIOLOGY BRUCELLA ABORTUS Brucellosis, caused by Brucella abortus is a major disease of cattle and a zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), a total 765 herds (13078 bovines) from six regions of CR were randomly sampled during 2012–2013. A non-random sample of 7907 herds (532199 bovines) of the six regions, arriving for diagnoses during 2014–2016 to the Costa Rican Animal Health Service was also studied. The prevalence estimated by Rose Bengal test (RBT) ranged from 10.5%-11.4%; alternatively, the prevalence estimated by testing the RBT positives in iELISA, ranged from 4.1%-6.0%, respectively. However, cattle in CR are not vaccinated with B. abortus S19 but with RB51 (vaccination coverage close to 11%), and under these conditions the RBT displays 99% specificity and 99% sensitivity. Therefore, the RBT herd depicted in the random analysis stands as a feasible assessment and then, the recommended value in case of planning an eradication program in CR. Studies of three decades reveled that bovine brucellosis prevalence has increased in CR. B. abortus was identified by biochemical and molecular studies as the etiological agent of bovine brucellosis. Multiple locus variable-number tandem repeat analysis-16 revealed four B. abortus clusters. Cluster one and three are intertwined with isolates from other countries, while clusters two and four have only representatives from CR. Cluster one is widely distributed in all regions of the country and may be the primary B. abortus source. The other clusters seem to be restricted to specific areas in CR. The implications of our findings, in relation to the control of the disease in CR, are critically discussed. La brucelosis, causada por Brucella abortus es una enfermedad importante del ganado y una zoonosis. En orden para estimar la prevalencia de la brucelosis bovina en Costa Rica (CR), un total de 765 rebaños (13078 bovinos) de seis regiones de la República Checa fueron objeto de un muestreo aleatorio durante 2012-2013. Una muestra no aleatoria muestra de 7907 rebaños (532199 bovinos) de las seis regiones, que llegaron para ser diagnosticados durante 2014-2016 al Servicio de Salud Animal de Costa Rica. La prevalencia estimada por la prueba de Rosa de Bengala (RBT) osciló entre el 10,5% y el 11,4%; alternativamente, la prevalencia estimada por las pruebas de los positivos de RBT en iELISA, osciló entre el 4,1% y el 6,0%, respectivamente. Sin embargo, los bovinos de la República Checa no están vacunados con B. abortus S19 sino con RB51 (cobertura de vacunación cercana al 11%), y bajo estas condiciones la RBT muestra un 99% de especificidad y un 99% de sensibilidad. Por lo tanto, el rebaño de RBT representado en el análisis aleatorio se mantiene como una evaluación factible y, a continuación, el valor recomendado en caso de planificar un programa de erradicación en CR. Los estudios de tres décadas revelaron que la prevalencia de la brucelosis bovina ha aumentado en CR. B. abortus fue identificado por estudios bioquímicos y moleculares como el agente etiológico de la brucelosis bovina. El análisis de repetición en tándem de número de variables de locus múltiples-16 reveló cuatro grupos de B. abortus. Los grupos uno y tres están entrelazados con aislamientos de otros países, mientras que los grupos dos y cuatro sólo tienen representantes de CR. El grupo uno está ampliamente distribuido en todas las regiones del país y puede ser la principal fuente de B. abortus. Los demás grupos parecen estar restringidos a zonas específicas de la República Checa. Las implicaciones de nuestros hallazgos, en relación con el control de la enfermedad en la RC, son discutidos críticamente. Este trabajo fue parcialmente financiado por Fondos del Sistema del Consejo Nacional de Rectores (FEES-CONARE FSI001) Costa Rica Reactivos y materiales. Las becas son las siguientes: GH-M fue patrocinado parcialmente por el proyecto de becas del Ministerio de Ciencia, Tecnología y Telecomunicaciones (MICITT-PINN) PND-033-15-2 y NR-V por el proyecto de becas de la Universidad de Costa Rica 803B4.5010, 17 -12-15. Los patrocinadores no participaron en el diseño del estudio, la recopilación y el análisis de datos, la decisión de publicar o la preparación del manuscrito. Escuela de Medicina Veterinaria 2020-10-14T00:53:31Z 2020-10-14T00:53:31Z 2017-08-10 http://purl.org/coar/resource_type/c_6501 http://hdl.handle.net/11056/18345 https://doi.org/10.1371/journal.pone.0182380 eng Acceso abierto Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf PLOS ONE PLoS ONE 12 (8): e0182380
institution Universidad Nacional de Costa Rica
collection Repositorio UNA-Costa Rica
language Inglés
topic COSTA RICA
BRUCELOSIS
BRUCELLA
EPIDEMIOLOGÍA
BOVINOS
ENFERMEDADES EN BOVINOS
EPIDEMIOLOGIA VETERINARIA
EPIDEMIOLOGY
CATTLE
DISEASES IN BOVINES
VETERINARY EPIDEMIOLOGY
BRUCELLA ABORTUS
spellingShingle COSTA RICA
BRUCELOSIS
BRUCELLA
EPIDEMIOLOGÍA
BOVINOS
ENFERMEDADES EN BOVINOS
EPIDEMIOLOGIA VETERINARIA
EPIDEMIOLOGY
CATTLE
DISEASES IN BOVINES
VETERINARY EPIDEMIOLOGY
BRUCELLA ABORTUS
Hernández-Mora, Gabriela
Ruiz-Villalobos, Nazareth
Bonilla-Montoya, Roberto
Romero-Zúñiga, Juan José
Jiménez-Arias, Julio
González-Barrientos, Rocio
BARQUERO-CALVO, ELIAS
Chacón-Díaz, Carlos
Rojas, Norman
Chaves-Olarte, Esteban
Guzman-Verri, Caterina
Moreno, Edgardo
Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease
description Brucellosis, caused by Brucella abortus is a major disease of cattle and a zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), a total 765 herds (13078 bovines) from six regions of CR were randomly sampled during 2012–2013. A non-random sample of 7907 herds (532199 bovines) of the six regions, arriving for diagnoses during 2014–2016 to the Costa Rican Animal Health Service was also studied. The prevalence estimated by Rose Bengal test (RBT) ranged from 10.5%-11.4%; alternatively, the prevalence estimated by testing the RBT positives in iELISA, ranged from 4.1%-6.0%, respectively. However, cattle in CR are not vaccinated with B. abortus S19 but with RB51 (vaccination coverage close to 11%), and under these conditions the RBT displays 99% specificity and 99% sensitivity. Therefore, the RBT herd depicted in the random analysis stands as a feasible assessment and then, the recommended value in case of planning an eradication program in CR. Studies of three decades reveled that bovine brucellosis prevalence has increased in CR. B. abortus was identified by biochemical and molecular studies as the etiological agent of bovine brucellosis. Multiple locus variable-number tandem repeat analysis-16 revealed four B. abortus clusters. Cluster one and three are intertwined with isolates from other countries, while clusters two and four have only representatives from CR. Cluster one is widely distributed in all regions of the country and may be the primary B. abortus source. The other clusters seem to be restricted to specific areas in CR. The implications of our findings, in relation to the control of the disease in CR, are critically discussed.
format Artículo
author Hernández-Mora, Gabriela
Ruiz-Villalobos, Nazareth
Bonilla-Montoya, Roberto
Romero-Zúñiga, Juan José
Jiménez-Arias, Julio
González-Barrientos, Rocio
BARQUERO-CALVO, ELIAS
Chacón-Díaz, Carlos
Rojas, Norman
Chaves-Olarte, Esteban
Guzman-Verri, Caterina
Moreno, Edgardo
author_sort Hernández-Mora, Gabriela
title Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease
title_short Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease
title_full Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease
title_fullStr Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease
title_full_unstemmed Epidemiology of bovine brucellosis in Costa Rica: Lessons learned from failures in the control of the disease
title_sort epidemiology of bovine brucellosis in costa rica: lessons learned from failures in the control of the disease
publisher PLOS ONE
publishDate 2020
url http://hdl.handle.net/11056/18345
https://doi.org/10.1371/journal.pone.0182380
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score 12.040022