Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3

Members of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of Brucella species that cause disease in mammals, including humans. A Brucella species, labeled as Brucella sp. BCCN84.3, was isolated from the testes of a Saint Be...

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Main Authors: Guzman-Verri, Caterina, Suárez-Esquivel, Marcela, Ruíz-Villalobos, Nazareth, Zygmunt, Michel S., Gonnet, Mathieu, Campos, Elena, Víquez-Ruiz, Eunice, Chacón-Díaz, Carlos, Aragón-Aranda, Beatriz, Conde-Álvarez, Raquel, Moriyon, Ignacio, Blasco, Jose-María, Muñoz, Pilar M., Baker, Kate, Thomson, Nicholas, Cloeckaert, Axel, Moreno, Edgardo
Format: Artículo
Language: Inglés
Published: Frontiers in Veterinary Science 2020
Subjects:
DOG
Online Access: http://hdl.handle.net/11056/18322
https://doi.org/10.3389/fvets.2019.00175
id RepoUNACR18322
recordtype dspace
spelling RepoUNACR183222020-10-10T09:01:50Z Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3 Guzman-Verri, Caterina Suárez-Esquivel, Marcela Ruíz-Villalobos, Nazareth Zygmunt, Michel S. Gonnet, Mathieu Campos, Elena Víquez-Ruiz, Eunice Chacón-Díaz, Carlos Aragón-Aranda, Beatriz Conde-Álvarez, Raquel Moriyon, Ignacio Blasco, Jose-María Muñoz, Pilar M. Baker, Kate Thomson, Nicholas Cloeckaert, Axel Moreno, Edgardo BRUCELLA BRUCELLA CANIS PERRO DOG BRUCELOSIS EPIDIDYMITIS Members of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of Brucella species that cause disease in mammals, including humans. A Brucella species, labeled as Brucella sp. BCCN84.3, was isolated from the testes of a Saint Bernard dog suffering orchiepididymitis, in Costa Rica. Following standard microbiological methods, the bacterium was first defined as “Brucella melitensis biovar 2.” Further molecular typing, identified the strain as an atypical “Brucella suis.” Distinctive Brucella sp. BCCN84.3 markers, absent in other Brucella species and strains, were revealed by fatty acid methyl ester analysis, high resolution melting PCR and omp25 and omp2a/omp2b gene diversity. Analysis of multiple loci variable number of tandem repeats and whole genome sequencing demonstrated that this isolate was different from the currently described Brucella species. The smooth Brucella sp. BCCN84.3 clusters together with the classical Brucella clade and displays all the genes required for virulence. Brucella sp. BCCN84.3 is a species nova taxonomical entity displaying pathogenicity; therefore, relevant for differential diagnoses in the context of brucellosis. Considering the debate on the Brucella species concept, there is a need to describe the extant taxonomical entities of these pathogens in order to understand the dispersion and evolution. Los miembros del género Brucella se agrupan en dos grupos filogenéticos: el clásico y el especies no clásicas. El primer grupo está compuesto por especies de Brucella que causan enfermedad en los mamíferos, incluyendo a los humanos. Una especie de Brucella, etiquetada como Brucella sp. BCCN84.3, fue aislado de los testículos de un perro de San Bernardo que sufría orquiepididimitis, en Costa Rica. Siguiendo los métodos microbiológicos estándar, la La bacteria fue definida por primera vez como "Brucella melitensis biovar 2". Más tipificación molecular, identificó la cepa como una "Brucella suis" atípica. Distintivo Brucella sp. BCCN84.3 los marcadores, ausentes en otras especies y cepas de Brucella, fueron revelados por el ácido graso análisis de éster de metilo, PCR de fusión de alta resolución y gen omp25 y omp2a/omp2b diversidad. Análisis de múltiples loci número variable de repeticiones en tándem y genoma completo La secuenciación demostró que este aislado era diferente del actualmente descrito La especie Brucella. La suave Brucella sp. BCCN84.3 se agrupa junto con la clásica Brucella clona y muestra todos los genes necesarios para la virulencia. Brucella sp. BCCN84.3 es una entidad taxonómica de la especie nova que muestra patogenicidad; por lo tanto, relevante para diagnósticos diferenciales en el contexto de la brucelosis. Considerando el debate sobre la Brucella concepto de especie, es necesario describir las entidades taxonómicas existentes de estas patógenos para entender la dispersión y la evolución. Universidad Nacional, Costa Rica Escuela de Medicina Veterinaria 2020-10-09T23:03:26Z 2020-10-09T23:03:26Z 2019-06-07 http://purl.org/coar/resource_type/c_6501 http://hdl.handle.net/11056/18322 https://doi.org/10.3389/fvets.2019.00175 eng Acceso abierto application/pdf Frontiers in Veterinary Science Front. Vet. Sci., 07 June 2019
institution Universidad Nacional de Costa Rica
collection Repositorio UNA-Costa Rica
language Inglés
topic BRUCELLA
BRUCELLA CANIS
PERRO
DOG
BRUCELOSIS
EPIDIDYMITIS
spellingShingle BRUCELLA
BRUCELLA CANIS
PERRO
DOG
BRUCELOSIS
EPIDIDYMITIS
Guzman-Verri, Caterina
Suárez-Esquivel, Marcela
Ruíz-Villalobos, Nazareth
Zygmunt, Michel S.
Gonnet, Mathieu
Campos, Elena
Víquez-Ruiz, Eunice
Chacón-Díaz, Carlos
Aragón-Aranda, Beatriz
Conde-Álvarez, Raquel
Moriyon, Ignacio
Blasco, Jose-María
Muñoz, Pilar M.
Baker, Kate
Thomson, Nicholas
Cloeckaert, Axel
Moreno, Edgardo
Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3
description Members of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed of Brucella species that cause disease in mammals, including humans. A Brucella species, labeled as Brucella sp. BCCN84.3, was isolated from the testes of a Saint Bernard dog suffering orchiepididymitis, in Costa Rica. Following standard microbiological methods, the bacterium was first defined as “Brucella melitensis biovar 2.” Further molecular typing, identified the strain as an atypical “Brucella suis.” Distinctive Brucella sp. BCCN84.3 markers, absent in other Brucella species and strains, were revealed by fatty acid methyl ester analysis, high resolution melting PCR and omp25 and omp2a/omp2b gene diversity. Analysis of multiple loci variable number of tandem repeats and whole genome sequencing demonstrated that this isolate was different from the currently described Brucella species. The smooth Brucella sp. BCCN84.3 clusters together with the classical Brucella clade and displays all the genes required for virulence. Brucella sp. BCCN84.3 is a species nova taxonomical entity displaying pathogenicity; therefore, relevant for differential diagnoses in the context of brucellosis. Considering the debate on the Brucella species concept, there is a need to describe the extant taxonomical entities of these pathogens in order to understand the dispersion and evolution.
format Artículo
author Guzman-Verri, Caterina
Suárez-Esquivel, Marcela
Ruíz-Villalobos, Nazareth
Zygmunt, Michel S.
Gonnet, Mathieu
Campos, Elena
Víquez-Ruiz, Eunice
Chacón-Díaz, Carlos
Aragón-Aranda, Beatriz
Conde-Álvarez, Raquel
Moriyon, Ignacio
Blasco, Jose-María
Muñoz, Pilar M.
Baker, Kate
Thomson, Nicholas
Cloeckaert, Axel
Moreno, Edgardo
author_sort Guzman-Verri, Caterina
title Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3
title_short Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3
title_full Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3
title_fullStr Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3
title_full_unstemmed Genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth Brucella sp. BCCN84.3
title_sort genetic and phenotypic characterization of the etiological agent of canine orchiepididymitis smooth brucella sp. bccn84.3
publisher Frontiers in Veterinary Science
publishDate 2020
url http://hdl.handle.net/11056/18322
https://doi.org/10.3389/fvets.2019.00175
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