Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues
Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, par...
| Autores Principales: | Morales, Juan Alberto, Soto, Esteban, Illanes, Oscar, Hilchie, David, Sunyakumthorn, Piyanate, Hawke, John, Goodwin, Andrew, Riggs, Allen, Yanong, Roy P., Pouder, Deborah B., Francis-Floyd, Ruth, Arauz, Maziel, Lewis Bogdanovic, Maziel Arauz,, Castillo-Alcala, Fernanda |
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| Formato: | Artículo |
| Idioma: | Inglés |
| Publicado: |
Journal of Veterinary Diagnostic Investigation
2020
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| Materias: | |
| Acceso en línea: |
http://jvdi.sagepub.com http://hdl.handle.net/11056/18041 |
| Sumario: |
Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, paraffin-embedded (FFPE) tissues from several fish species, collected at different geographical locations from 2000 to 2011, were analyzed using a real-time polymerase chain reaction assay for the detection of the Fno intracellular growth loci C (iglC) gene and by immunohistochemistry for the demonstration of Fno antigens. The results revealed a high correlation between these 2 diagnostic techniques validating their use for the diagnosis of Fno infection in archived FFPE tissues and confirming the presence of Fno in fish species from the Cari y years of the present century. |
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