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Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L)

Among the vegetables, the onion (Allium cepa L.) is the second largest consumed crop in the world and in 2015, Costa Rica planted approximately 1633.6 ha of onion. This production is affected by the presence of phytopathogens, especially fungi that attack roots such as Setophoma terrestris, which ca...

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Autores Principales: López-Courrau, Karol, Rivera-Méndez, William, Brenes-Madriz, Jaime, Zúñiga-Vega, Claudia
Formato: Artículo
Idioma: Español
Publicado: Editorial Tecnológica de Costa Rica (entidad editora) 2018
Materias:
Fusarium sp
Setophoma terrestris
cebolla
ajo
Acceso en línea: https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3958
https://hdl.handle.net/2238/11824
id RepoTEC11824
recordtype dspace
spelling RepoTEC118242020-09-25T23:12:04Z Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L) Establecimiento de un protocolo para el crecimiento y multiplicación de Setophoma terrestris y Fusarium spp. provenientes de un cultivo de cebolla (Allium cepa L) López-Courrau, Karol Rivera-Méndez, William Brenes-Madriz, Jaime Zúñiga-Vega, Claudia Fusarium sp Setophoma terrestris cebolla ajo Fusarium sp Setophoma terrestris cebolla ajo Among the vegetables, the onion (Allium cepa L.) is the second largest consumed crop in the world and in 2015, Costa Rica planted approximately 1633.6 ha of onion. This production is affected by the presence of phytopathogens, especially fungi that attack roots such as Setophoma terrestris, which causes the disease known as pink root, and the fungi of the genus Fusarium spp. that causes basal rot. The objective of this research was to establish a protocol to optimize the growth of both fungi (S. terrestris and Fusarium spp.) in liquid medium, in order to facilitate their identification, manipulation and subsequent experimental works. Various physical parameters (pH, temperature, stirring speed and photoperiod) were evaluated and different culture media compositions were tested. The culture media analyzed in Fusarium spp. were potato dextrose homemade and commercial, oats, V8, SNA, Sabouraud dextrose and water. In addition, potato dextrose commercial and homemade, oats, vainica, minimum medium and water were tested for S. terrestris. Differences in treatments were determined using an analysis of variance and the Tukey test. This study demonstrated that Fusarium spp. grows optimally at 30 °C, pH 6, 100 rpm and darkness, and S. terrestris does at 28°C, pH 4, 100 rpm. In this fungus, growth under darkness stimulated biomass gain whereas light incidence resulted in reproductive phase establishment. The ideal medium for S. terrestris was the vainica for reproduction and homemade potato dextrose for vegetative growth, meanwhile for Fusarium spp. homemade dextrose potato medium was optimal for both processes. La cebolla (Allium cepa L.) ocupa el segundo lugar de consumo de hortalizas en el mundo y en Costa Rica, en el 2015, se cultivaron 1633,6 ha aproximadamente. Este cultivo se puede ver afectado por la presencia de fitopatógenos, especialmente los hongos que atacan las raíces como Setophoma terrestris, que ocasiona la enfermedad conocida como raíz rosada, y el género Fusarium spp. que provoca la podredumbre basal. El objetivo de esta investigación consistió en establecer un protocolo para la optimización del crecimiento y la multiplicación de S. terrestris y Fusarium spp. en medio líquido, para facilitar su identificación, manipulación y trabajos experimentales posteriores. Para esto se realizó la evaluación de diferentes parámetros físicos (pH, temperatura, velocidad de agitación y fotoperiodo) y de distintos medios de cultivo. Los medios analizados para Fusarium spp. fueron papa dextrosa casero y comercial, avena, V8, SNA, Sabouraud dextrosa y agua. En S. terrestris se evaluaron papa dextrosa comercial y casero, avena, vainica, medio mínimo y agua. Las diferencias de los tratamientos se determinaron mediante un análisis de varianza y la prueba Tukey. Se concluyó que Fusarium spp. crece óptimamente a 30°C, pH 6, 100 rpm y oscuridad, mientras que S. terrestris lo hace a 28°C, pH 4, 100 rpm. En este último, el crecimiento en oscuridad afectó positivamente el incremento de biomasa, mientras que la luz continua indujo el desarrollo de estructuras reproductivas. El medio de cultivo idóneo para S. terrestris fue el de vainica para reproducción y papa dextrosa casero para biomasa, y para Fusarium spp. fue papa dextrosa casero para ambos procesos (biomasa y reproducción). 2018-10-03 2020-09-25T23:12:04Z 2020-09-25T23:12:04Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3958 10.18845/tm.v31i4.3958 https://hdl.handle.net/2238/11824 spa https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3958/3546 application/pdf Editorial Tecnológica de Costa Rica (entidad editora) Tecnología en marcha Journal; Vol. 31, Núm. 4: Octubre-Noviembre 2018; pág. 37-48 Revista Tecnología en Marcha; Vol. 31, Núm. 4: Octubre-Noviembre 2018; pág. 37-48 2215-3241 0379-3982
institution Tecnológico de Costa Rica
collection Repositorio TEC
language Español
topic Fusarium sp
Setophoma terrestris
cebolla
ajo
Fusarium sp
Setophoma terrestris
cebolla
ajo
spellingShingle Fusarium sp
Setophoma terrestris
cebolla
ajo
Fusarium sp
Setophoma terrestris
cebolla
ajo
López-Courrau, Karol
Rivera-Méndez, William
Brenes-Madriz, Jaime
Zúñiga-Vega, Claudia
Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L)
description Among the vegetables, the onion (Allium cepa L.) is the second largest consumed crop in the world and in 2015, Costa Rica planted approximately 1633.6 ha of onion. This production is affected by the presence of phytopathogens, especially fungi that attack roots such as Setophoma terrestris, which causes the disease known as pink root, and the fungi of the genus Fusarium spp. that causes basal rot. The objective of this research was to establish a protocol to optimize the growth of both fungi (S. terrestris and Fusarium spp.) in liquid medium, in order to facilitate their identification, manipulation and subsequent experimental works. Various physical parameters (pH, temperature, stirring speed and photoperiod) were evaluated and different culture media compositions were tested. The culture media analyzed in Fusarium spp. were potato dextrose homemade and commercial, oats, V8, SNA, Sabouraud dextrose and water. In addition, potato dextrose commercial and homemade, oats, vainica, minimum medium and water were tested for S. terrestris. Differences in treatments were determined using an analysis of variance and the Tukey test. This study demonstrated that Fusarium spp. grows optimally at 30 °C, pH 6, 100 rpm and darkness, and S. terrestris does at 28°C, pH 4, 100 rpm. In this fungus, growth under darkness stimulated biomass gain whereas light incidence resulted in reproductive phase establishment. The ideal medium for S. terrestris was the vainica for reproduction and homemade potato dextrose for vegetative growth, meanwhile for Fusarium spp. homemade dextrose potato medium was optimal for both processes.
format Artículo
author López-Courrau, Karol
Rivera-Méndez, William
Brenes-Madriz, Jaime
Zúñiga-Vega, Claudia
author_sort López-Courrau, Karol
title Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L)
title_short Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L)
title_full Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L)
title_fullStr Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L)
title_full_unstemmed Establishment of a protocol for the growth and multiplication of Setophoma terrestris and Fusarium spp. from an onion culture (Allium cepa L)
title_sort establishment of a protocol for the growth and multiplication of setophoma terrestris and fusarium spp. from an onion culture (allium cepa l)
publisher Editorial Tecnológica de Costa Rica (entidad editora)
publishDate 2018
url https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3958
https://hdl.handle.net/2238/11824
_version_ 1796140754279596032
score 12.041432

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