Optimization of a metabolic pathway in Escherichia coli for the production of farnesene through the use of synthetic biology

Interest compounds generation using organic waste as raw material results in an economically and industrially viable option. Through synthetic biology is possible the design of microorganisms capable of using these residues as carbon sources in order to produce biofuels, vaccines, alcohol, oils and...

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Main Authors: Garro-Monge, Giovanni, García-Gómez, David, Valerín-Berrocal, Karla, Jiménez-Quesada, Karol
Format: Artículo
Language: Español
Published: Editorial Tecnológica de Costa Rica (entidad editora) 2018
Subjects:
Online Access: https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3957
https://hdl.handle.net/2238/11823
Summary: Interest compounds generation using organic waste as raw material results in an economically and industrially viable option. Through synthetic biology is possible the design of microorganisms capable of using these residues as carbon sources in order to produce biofuels, vaccines, alcohol, oils and other products. Farnesene production and its precursors is possible through the genetic modification of microorganisms like Escherichia coli, introducing metabolic pathways capable of produce the interest compounds. After studying, optimizing and synthesizing the genes that make up the metabolic pathway of mevalonate, which originates farnesene, these were assembled together with several Anderson family promoters to transform E. coli and generate E, E, -farnesene from synthetic form.