Vista Equipo: Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity

Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity

Plants of the genus Phyllanthus are currently studied because of their ability to produce phenolic compounds with high antioxidant capacity. Additionally, several studies have demonstrated their capability to induce cellular apoptosis through secondary metabolites (phyllanthostatin), which provides...

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Autores Principales:	Garro-Monge, Giovanni, Jiménez-Quesada, Karol, Pérez-Méndez, Raquel
Formato:	Artículo
Idioma:	Español
Publicado:	Editorial Tecnológica de Costa Rica (entidad editora) 2018
Materias:	Genetic transformation agro-infection bacterial strain secondary metabolism microscopy. Transformación genética agro-infección cepa bacteriana metabolismo secundario microscopía.
Acceso en línea:	https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3903 https://hdl.handle.net/2238/11814

id	RepoTEC11814
recordtype	dspace
spelling	RepoTEC118142020-09-25T23:12:03Z Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity Desarrollo de un sistema de cultivo in vitro de raíces pilosas de Phyllantus acumminatus, para la producción de compuestos con actividad biológica Garro-Monge, Giovanni Jiménez-Quesada, Karol Pérez-Méndez, Raquel Genetic transformation agro-infection bacterial strain secondary metabolism microscopy. Transformación genética agro-infección cepa bacteriana metabolismo secundario microscopía. Plants of the genus Phyllanthus are currently studied because of their ability to produce phenolic compounds with high antioxidant capacity. Additionally, several studies have demonstrated their capability to induce cellular apoptosis through secondary metabolites (phyllanthostatin), which provides these plants a great potential for possible cancer prevention treatments. In this research, P. accuminatus seeds were introduced in vitro, in vitro culture of the seedlings was achieved, and the vitroplants were used in order to induce hairy roots by genetic transformation with Ar15834 of Agrobacterium rhizogenes. The most promising assay for the induction of these organogenic cultures consisted of the use of 1-2 cm stems to which transverse wounds were made in order to inoculate them in the bacterial culture (OD600: 0.6-1). The stems were placed in hormone-free semisolid M & S medium containing 50% of salts and vitamins during 48 hours. After this time, the stems were transferred to a hormone-free semisolid 100% M & S medium supplemented with Ceftriaxona® (500 µg/l) to remove the bacteria. Finally, the hairy roots cultures were accomplished by using hormone-free semisolid 100% M & S medium. Besides, through light microscopy, the established roots morphologic characterization was carried out and it matched the typical hairy roots morphology reported when agro-infection system is used. Las plantas del género Phyllanthus son actualmente investigadas por su habilidad para producir compuestos fenólicos con alta capacidad antioxidante. Además, diversos estudios han revelado que estas plantas poseen metabolitos (filantósidos y lignanos) que pueden inducir la apoptosis celular, lo que les proporciona un gran potencial para posibles tratamientos de prevención contra el cáncer. En este trabajo, se llevó a cabo la introducción in vitro de semillas de P. acumminatus; se logró el cultivo in vitro del material vegetal, y las vitro-plantas fueron utilizadas en ensayos de inducción de raíces pilosas, mediante transformación genética con Agrobacterium rhizogenes cepa Ar15834. El tratamiento más favorable para la inducción de estos cultivos organogénicos consistió en el uso de estacas de aproximadamente 1-2 cm, a las cuales se les hicieron cortes transversales con un bisturí, para luego ser inoculadas en el cultivo bacteriano (OD600: 0.6-1). Las estacas fueron colocadas en medio M & S semisólido al 50% de sales y vitaminas, sin reguladores de crecimiento, por un período de 48 h. Transcurrido este tiempo, las estacas fueron micropropagadas en medio M & S al 100% de sales y vitaminas, complementado con 500 µg/l del antibiótico Ceftriaxona® para erradicar la bacteria. Se logró el cultivo de las raíces pilosas en medio M & S al 100% de sales y vitaminas, sin reguladores de crecimiento. Por medio del uso de microscopía de luz, se logró la caracterización morfológica de las raíces establecidas, las cuales coinciden plenamente con las típicas raíces pilosas o hairy roots obtenidas por medio del sistema de agro-infección, según la literatura consultada. 2018-07-11 2020-09-25T23:12:03Z 2020-09-25T23:12:03Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3903 10.18845/tm.v31i3.3903 https://hdl.handle.net/2238/11814 spa https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3903/pdf application/pdf Editorial Tecnológica de Costa Rica (entidad editora) Tecnología en marcha Journal; Vol. 31, Núm. 3: Julio-Setiembre 2018; pág. 66-73 Revista Tecnología en Marcha; Vol. 31, Núm. 3: Julio-Setiembre 2018; pág. 66-73 2215-3241 0379-3982
institution	Tecnológico de Costa Rica
collection	Repositorio TEC
language	Español
topic	Genetic transformation agro-infection bacterial strain secondary metabolism microscopy. Transformación genética agro-infección cepa bacteriana metabolismo secundario microscopía.
spellingShingle	Genetic transformation agro-infection bacterial strain secondary metabolism microscopy. Transformación genética agro-infección cepa bacteriana metabolismo secundario microscopía. Garro-Monge, Giovanni Jiménez-Quesada, Karol Pérez-Méndez, Raquel Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity
description	Plants of the genus Phyllanthus are currently studied because of their ability to produce phenolic compounds with high antioxidant capacity. Additionally, several studies have demonstrated their capability to induce cellular apoptosis through secondary metabolites (phyllanthostatin), which provides these plants a great potential for possible cancer prevention treatments. In this research, P. accuminatus seeds were introduced in vitro, in vitro culture of the seedlings was achieved, and the vitroplants were used in order to induce hairy roots by genetic transformation with Ar15834 of Agrobacterium rhizogenes. The most promising assay for the induction of these organogenic cultures consisted of the use of 1-2 cm stems to which transverse wounds were made in order to inoculate them in the bacterial culture (OD600: 0.6-1). The stems were placed in hormone-free semisolid M & S medium containing 50% of salts and vitamins during 48 hours. After this time, the stems were transferred to a hormone-free semisolid 100% M & S medium supplemented with Ceftriaxona® (500 µg/l) to remove the bacteria. Finally, the hairy roots cultures were accomplished by using hormone-free semisolid 100% M & S medium. Besides, through light microscopy, the established roots morphologic characterization was carried out and it matched the typical hairy roots morphology reported when agro-infection system is used.
format	Artículo
author	Garro-Monge, Giovanni Jiménez-Quesada, Karol Pérez-Méndez, Raquel
author_sort	Garro-Monge, Giovanni
title	Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity
title_short	Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity
title_full	Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity
title_fullStr	Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity
title_full_unstemmed	Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity
title_sort	development of phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity
publisher	Editorial Tecnológica de Costa Rica (entidad editora)
publishDate	2018
url	https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/3903 https://hdl.handle.net/2238/11814
_version_	1796139185824858112
score	12.041337

Development of Phyllantus acumminatus hairy roots culture system for the production of compounds with biological activity

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