Staff View: Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom

Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom

artículo (preprint) -- Universidad de Costa Rica, Instituto de Investigaciones Clodomiro Picado, 2014

Main Authors:	Lomonte, Bruno, Pla Ferrer, Davinia, Sasa Marín, Mahmood, Tsai, Wan-Chih, Solórzano López, Alejandro, Ureña Diaz, Juan M., Fernández Montes de Oca, María Laura, Mora Obando, Diana, Sanz, Libia, Gutiérrez, José María, Calvete Chornet, Juan José
Format:	Preprint
Language:	Inglés
Published:	Journal of Proteomics Volume 103, 30 May 2014, Pages 137–152 2014
Subjects:	Snake venom Veneno de serpiente Proteomics Venomics Pelamis
Online Access:	http://www.sciencedirect.com/science/article/pii/S1874391914001560 https://hdl.handle.net/10669/11047

id	11047
recordtype	dspace
spelling	110472021-03-30T18:32:58Z Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom Lomonte, Bruno Pla Ferrer, Davinia Sasa Marín, Mahmood Tsai, Wan-Chih Solórzano López, Alejandro Ureña Diaz, Juan M. Fernández Montes de Oca, María Laura Mora Obando, Diana Sanz, Libia Gutiérrez, José María Calvete Chornet, Juan José Snake venom Veneno de serpiente Proteomics Venomics Pelamis artículo (preprint) -- Universidad de Costa Rica, Instituto de Investigaciones Clodomiro Picado, 2014 The yellow-bellied sea snake, Pelamis platura, is the most broadly distributed snake species. Despite being endowed with a highly lethal venom, a proteomic analysis of its toxin composition was unavailable. The venoms of specimens collected in Golfo de Papagayo and Golfo Dulce (Costa Rica), where two distinctive color morphs occur, were chromatographically compared. The latter inhabits a fjord-like gulf where the transit of oceanic sea snakes into and from the basin is restricted, thus possibly affecting gene flow. RP-HPLC evidenced a conserved venom protein profile in both populations, despite their divergent color phenotypes. Following a trend observed in other sea snakes, P. platura venom is relatively simple, being composed of proteins of the three-finger toxin (3FTx), phospholipase A2 (PLA2), cysteine-rich secretory protein (CRISP), 5′-nucleotidase, and metalloproteinase families. The first three groups represent 49.9%, 32.9%, and 9.1% of total venom protein, respectively. The most abundant component (~ 26%) is pelamitoxin (P62388), a short-chain 3FTx, followed by a major basic PLA2 (~ 20%) and a group of three isoforms of CRISPs (~ 9%). Whereas isolated pelamitoxin was highly lethal to mice, neither the PLA2 nor the CRISP fraction caused death. However, the PLA2 rapidly increased plasma creatine kinase activity after intramuscular injection, indicating its myotoxic action. Differing from myotoxic PLA2s of viperids, this PLA2 was not cytolytic to murine myogenic cells in vitro, suggesting possible differences in its mechanism of action. The median lethal dose (LD50) estimates for P. platura crude venom in mice and in three species of fishes did not differ significantly. The sea snake antivenom manufactured by CSL Ltd. (Australia), which uses Enhydrina schistosa as immunogen, cross-recognized the three major components of P. platura venom and, accordingly, neutralized the lethal activity of crude venom and pelamitoxin, therefore being of potential usefulness in the treatment of envenomations by this species. Funded by grants from Ministerio de Economía y Competitividad, Madrid, BFU2010-17373; PROMETEO/2010/005 from the Generalitat Valenciana, Spain; Vicerrectoría de Investigación, Universidad de Costa Rica (741-B2-652 and 741-B3-760; Network for proteomic characterization of snake venoms of medical and biological relevance in Latin America), and CYTED (project BioTox, P211RT0412). UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) 2014-05-02T21:52:29Z 2014-05-02T21:52:29Z 2014-05-30 preprint http://www.sciencedirect.com/science/article/pii/S1874391914001560 1874-3919 essn:1876-7737 https://hdl.handle.net/10669/11047 10.1016/j.jprot.2014.03.034 741-B2-652 741-B3-760 en_US Atribución-NoComercial-SinDerivadas 3.0 Costa Rica http://creativecommons.org/licenses/by-nc-nd/3.0/cr/ application/pdf Journal of Proteomics Volume 103, 30 May 2014, Pages 137–152
institution	Universidad de Costa Rica
collection	Repositorio KERWA
language	Inglés
topic	Snake venom Veneno de serpiente Proteomics Venomics Pelamis
spellingShingle	Snake venom Veneno de serpiente Proteomics Venomics Pelamis Lomonte, Bruno Pla Ferrer, Davinia Sasa Marín, Mahmood Tsai, Wan-Chih Solórzano López, Alejandro Ureña Diaz, Juan M. Fernández Montes de Oca, María Laura Mora Obando, Diana Sanz, Libia Gutiérrez, José María Calvete Chornet, Juan José Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom
description	artículo (preprint) -- Universidad de Costa Rica, Instituto de Investigaciones Clodomiro Picado, 2014
format	Preprint
author	Lomonte, Bruno Pla Ferrer, Davinia Sasa Marín, Mahmood Tsai, Wan-Chih Solórzano López, Alejandro Ureña Diaz, Juan M. Fernández Montes de Oca, María Laura Mora Obando, Diana Sanz, Libia Gutiérrez, José María Calvete Chornet, Juan José
author_sort	Lomonte, Bruno
title	Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom
title_short	Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom
title_full	Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom
title_fullStr	Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom
title_full_unstemmed	Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom
title_sort	two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of costa rica: snake venomics, toxicity, and neutralization by antivenom
publisher	Journal of Proteomics Volume 103, 30 May 2014, Pages 137–152
publishDate	2014
url	http://www.sciencedirect.com/science/article/pii/S1874391914001560 https://hdl.handle.net/10669/11047
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score	11.959784

Two color morphs of the pelagic yellow-bellied sea snake, pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom

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