Conditioning factors that influence the spontaneous voltage gradient and the transepithelial resistence in diverse in vitro mammalians and amphibians epithelia
An experimental study about spontaneous voltage gradient, total transepithelial resistance and short-circuit current is made on different amphibian and mammalian epithelia. The dissected tissue is placed between two cubic chambers. The biological membrane divides two electrolytic oxigenated-solution...
| Autor Principal: | Serrano C., Eric J. |
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| Formato: | Artículo |
| Idioma: | Español |
| Publicado: |
2004
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| Materias: | |
| Acceso en línea: |
http://up-rid.up.ac.pa/848/ http://up-rid.up.ac.pa/848/1/Tecnociencia%20Articulo%207%206%282%29%2004.pdf |
| Sumario: |
An experimental study about spontaneous voltage gradient, total transepithelial
resistance and short-circuit current is made on different amphibian and mammalian
epithelia. The dissected tissue is placed between two cubic chambers. The
biological membrane divides two electrolytic oxigenated-solutions at 20°C and pH
7.4 conditions. A multimeter measures the voltage difference between the solutions,
then 6 for transepithelial resistance
calculation. Thereafter a 9 – 56 V outer electromotive force and a potential divider
are placed in series with the tissue to adjust and maintain zero potential, in order to
measure the short-circuit current. Significant differences with 0.01level confidence
were found in the electric epithelial parameters of the frog skin and urinary bladder
and in the rabbit and rat proximal colon, urinary bladder and gallbladder. While
[Na+
] decrease from 107 to 0.375 mM minimized the short-circuit current in the frog
skin, 100 mU/ml vasopressin in the serosal solution significantly increased it by
62%. The frog urinary bladder transepithelial resistance increased by means of pH
decrease from 7.4 to 6.0 in the serosal solution and also by 10 mM amiloride in the
mucosal solution. Neither the total transepithelial resistance nor the shor-circuit
current significantly changed in the rabbit urinary bladder exposed to amiloride. |
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