REPRODUCTIVE CONDITION ASSOCIATED TO CHANGES IN THE LIPID-SOLUBLE ANTIOXIDANT CAPACITY AND DAMAGE TO LIPIDS IN THE SEA URCHINS LOXECHINUS ALBUS (ECHINODERMATA: ECHINOIDEA)

The hypothesis of this study was that oxidative damage in the lipid phase of the gonad of the sea urchin Loxechinus albus increased during gametogenesis. Six different male and female reproductive conditions (reproductive stages) were described: (a) immature, (b) growing, (c) premature I, (d) premat...

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Main Authors: Pérez, Analía F.; Department of Ecology, Genetics and Evolution, School of Exact and Natural Sciences, University of Buenos Aires, Buenos Aires, Argentina., Malanga, Gabriela; Physical Chemistry-PRALIB, School of Pharmacy and Biochemistry, University of Buenos Aires-CONICET, Junín 956, 1113 Buenos Aires, Argentina., Puntarulo, Susana; Physical Chemistry-PRALIB, School of Pharmacy and Biochemistry, University of Buenos Aires-CONICET, Junín 956, 1113 Buenos Aires, Argentina.
Format: Artículo
Language: Español
Published: Universidad Nacional de Costa Rica, EUNA 2012
Subjects:
Online Access: http://www.revistas.una.ac.cr/index.php/revmar/article/view/3304
http://hdl.handle.net/11056/8169
Summary: The hypothesis of this study was that oxidative damage in the lipid phase of the gonad of the sea urchin Loxechinus albus increased during gametogenesis. Six different male and female reproductive conditions (reproductive stages) were described: (a) immature, (b) growing, (c) premature I, (d) premature II, (e) mature, and (f) spawned. The content of the lipid-soluble antioxidants, as well as the damage to lipids (assessed as the content of 2-thiobarbituric acid reactive substances (TBARS) and the content of lipid radicals) were analyzed. The content of the lipid-soluble antioxidants α-tocopherol (α-TH), β-carotene, and echinenone decreased during gametogenesis. In contrast, the content of both TBARS and lipid radicals increased. However, the consumption of lipid-soluble antioxidants was not sufficient to efficiently control lipid damage since the ratio of TBARS content/ α-TH content, taken as an index of damage/protection ratio, significantly increased during the mature stage. Therefore, an active food intake to incorporate antioxidants to the diet is required to adequately prepare the gonad for the next reproductive cycle.La hipótesis de este estudio fue que el daño oxidativo en la fase lipídica de las gónadas en el erizo Loxechinus albus se incrementa durante la gametogénesis. Se describieron seis condiciones reproductivas (estadios reproductivos) en machos y hembras: (a) inmaduros, (b) crecimiento, (c) premaduro I, (d) premaduro II, (e) maduro y (f) desove. El contenido de los antioxidantes liposolubles, así como el daño a lípidos (estimados como el contenido de sustancias reactivas al ácido 2-tiobarbitúrico (TBARS), y el contenido de radicales lipídicos) fue analizado. El contenido de los antioxidantes liposolubles α-tocoferol (α-TH), β-caroteno y equinenona disminuyó durante la gametogénesis. En contraste, tanto el contenido de TBARS como de los radicales lipídicos aumentó. Sin embargo, el consumo de antioxidantes liposolubles no resultó suficiente para controlar en forma eficiente el daño a lípidos; ya que el índice contenido de TBARS/contenido de α-TH,  tomado como un indicador de la relación daño/protección, aumentó significativamente durante la madurez gonadal. Por lo tanto, una activa alimentación que permita la incorporación de antioxidantes mediante la dieta se requiere para preparar adecuadamente a la gónada para el próximo ciclo reproductivo.