Antivenomic Characterization of Two Antivenoms Against the Venom of the Taipan, Oxyuranus scutellatus, from Papua New Guinea and Australia

Antivenoms manufactured by bioCSL Limited (Australia) and Instituto Clodomiro Picado (Costa Rica) against the venom of the taipan snakes (Oxyuranus scutellatus) from Australia and Papua New Guinea (PNG), respectively, were compared using antivenomics, an analytical approach that combines proteomics...

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Main Authors: Herrera Vega, María, Paiva, Owen K., Pagotto, Ana Helena, Segura Ruiz, Álvaro, Serrano, Solange M. T., Vargas Arroyo, Mariángela, Villalta Arrieta, Mauren, Jensen, Simon D., León Montero, Guillermo, Williams, David J., Gutiérrez, José María
Format: Artículo
Language: Inglés
Published: 2017
Subjects:
Online Access: http://www.ajtmh.org/content/91/5/887
http://hdl.handle.net/10669/30100
Summary: Antivenoms manufactured by bioCSL Limited (Australia) and Instituto Clodomiro Picado (Costa Rica) against the venom of the taipan snakes (Oxyuranus scutellatus) from Australia and Papua New Guinea (PNG), respectively, were compared using antivenomics, an analytical approach that combines proteomics with immunoaffinity chromatography. Both antivenoms recognized all venom proteins present in venom from PNG O. scutellatus, although a pattern of partial recognition was observed for some components. In the case of the Australian O. scutellatus venom, both antivenoms immunorecognized the majority of the components, but the CSL antivenom showed a stronger pattern of immunoreactivity, which was revealed by the percentage of retained proteins in the immunoaffinity column. Antivenoms interacted with taipoxin in surface plasmon resonance. These observations on antivenomics agree with previous neutralization studies.