Propagation of Dendrobium aggregatum by green capsule culture

An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP...

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Autores Principales: Vijayakumar, S., Rajalkshmi, G., Kalimuthu, K.
Formato: Artículo
Idioma: Inglés
Publicado: Universidad de Costa Rica 2013
Materias:
Acceso en línea: http://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/11763
http://hdl.handle.net/10669/20990
Sumario: An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.