A novel seed baiting technique for the epiphytic orchid Rhynchostele cervantesii, a means to acquire mycorrhizal fungi from protocorms

We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protoco...

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Autores Principales: Cruz Higareda, Jesús Bernardo, Luna Rosales, Bárbara Susana, Barba Álvarez, Amadeo
Formato: Artículo
Idioma: Español
Publicado: Universidad de Costa Rica 2015
Materias:
Acceso en línea: http://revistas.ucr.ac.cr/index.php/lankesteriana/article/view/18525
http://hdl.handle.net/10669/20977
Sumario: We developed a new and novel seed baiting technique sowing mature seeds of the epiphyitic orchid Rhynchostele cervantesii under natural conditions, We introduced a sponge in each package that may serve as a reservoir for water retention to benefit germination; In three of 22 packets we found protocorms in an early stage of development, six of wich were of sufficient size to warrant fungal isolations; Nine strains were isolated in pure culture and were inoculated on seeds and protocorms under in vitro conditions.