Conditioning of mealybug (Hemiptera: Pseudococcidae) by Scanning Electron Microscopy.

The aim of this work was to determine the methodology for an adequate conditioning for the cleaning of mealybugs specimens and its correct observation. This work was done in the laboratory of the Research Center in Microscopic Structures (CIEMIC) of the University of Costa Rica, in 2012. Four types...

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Main Authors: Palma Jiménez, Melissa, Blanco Meneses, Mónica
Format: Artículo
Language: Español
Published: Universidad de Costa Rica 2015
Online Access: http://revistas.ucr.ac.cr/index.php/agromeso/article/view/19330
http://hdl.handle.net/10669/17784
Summary: The aim of this work was to determine the methodology for an adequate conditioning for the cleaning of mealybugs specimens and its correct observation. This work was done in the laboratory of the Research Center in Microscopic Structures (CIEMIC) of the University of Costa Rica, in 2012. Four types of methodologies were implemented, which evidenced a gradual improvement of the observation of the ultrastructures through the Scanning Electron Microscopy. Every process was described in detail. The best results were showed with 10% xylene (in some cases it was feasible using 95-100% ethanol). It allowed to remove the wax from the body of the insect, avoiding its collapse, and observing the specific ultrastructures of the individual. This approach will reduce the time and cost of future taxonomic research of mealybugs.