Affinity and effect of Azospirillum sp. in corn.

Theobjective of this work was to determine the affi nity andeffect of Azospirillum in corn. This bioassay was conductedin the state of Mexico, México, during the spring – summercycle of 2009. The root system of corn Palomero Toluqueño,Cacahuacintle, Cónico, Chalqueño and H-20, and of Teocintle,was c...

Descripción completa

Autores Principales: Rangel Lucio, José Antonio, Rodríguez Mendoza, María De las Nieves, Ferrera Cerrato, Ronald, Castellanos Ramos, Javier Zaragoza, Ramírez Gama, Ana María, Alvarado Bárcenas, Estéfana
Formato: Artículo
Idioma: Español
Publicado: Universidad de Costa Rica 2011
Acceso en línea: http://revistas.ucr.ac.cr/index.php/agromeso/article/view/8689
http://hdl.handle.net/10669/17670
id RepoKERWA17670
recordtype dspace
spelling RepoKERWA176702017-08-08T18:49:41Z Affinity and effect of Azospirillum sp. in corn. Afinidad y efecto de Azospirillum sp. en maíz. Rangel Lucio, José Antonio Rodríguez Mendoza, María De las Nieves Ferrera Cerrato, Ronald Castellanos Ramos, Javier Zaragoza Ramírez Gama, Ana María Alvarado Bárcenas, Estéfana Theobjective of this work was to determine the affi nity andeffect of Azospirillum in corn. This bioassay was conductedin the state of Mexico, México, during the spring – summercycle of 2009. The root system of corn Palomero Toluqueño,Cacahuacintle, Cónico, Chalqueño and H-20, and of Teocintle,was collected, and after isolation and purifi cation ofstrains outstanding in their nitrogenasa activity (N’asa), werereinoculated aseptically in the same varieties at the seedlingstage (13 days) under hydroponic culture. Root volume wasdetermined, and also used to determine bacterial populationsand N’asa at 60 dde, in corn and Teocintle. Azospirillum didnot affect root volumen in relation to nitrogen fertilization,but the comparative study showed that strains isolated fromCónico and Chalqueño corn, in addition to Teocintle, formedmore root tissue in Chalqueño corn. Despite that the largestbacterial population was obtained with the strain isolatedfrom H-28 ans inoculated on corns Chalqueño, H-28 andCónico, the highest N’asa was recorded with strains isolatedfrom H-28, Palomero Toluqueño and Chalqueño, inoculatedon corn Chalqueño. There was a degree of affi nity, or effectof the homologous strains, between Azospirillum obtainedfrom corns H-28 and Chalqueño and their re-inoculation onthe same varieties, evaluating the number of azospirills andN’asa, and a survey of Azospirillum from recent corns, forancestors of the same cereal. Elobjetivo del presente trabajo fue determinar la afi nidad yefecto de cepas de Azospirillum en maíz. Este bioensayo sedesarrolló en el estado de México, México, durante el cicloprimavera - verano del 2009. Para ello se colectó la raíz demaíz Palomero Toluqueño, Cacahuacintle, Cónico, Chalqueñoy H-20, y de Teocintle, y después de aislar y purifi car lascepas sobresalientes por actividad nitrogenasa (N’asa), sere-inocularon ascépticamente en las mismas variedades enestadío de plántula de 13 días de edad (dde) en hidroponía.Se midió el volumen de la raíz, que también se acondicionópara determinar la población bacteriana y N’asa a los 60 ddede maíces y Teocintle. Azospirillum no infl uyó en el volumenradical en relación con la fertilización nitrogenada, peroel estudio comparativo demostró que las cepas aisladas demaíces Cónico y Chalqueño, además de Teocintle, formaronmayor tejido radical en maíz Chalqueño. A pesar de que lamayor población bacteriana se obtuvo con la cepa aislada deH-28 inoculada en maíces Chalqueño, H-28 y Cónico, losmayores registros de N’asa ocurrieron con cepas provenientesde H-28, Palomero Toluqueño y Chalqueño inoculadasen maíz Chalqueño. Hubo algún grado de afi nidad, o efectode la cepa homóloga, entre Azospirillum obtenida de maícesH-28 y Chalqueño y su reinoculación en estas mismas variedades,al evaluar el número de azospirilas y N’asa y unreconocimiento de Azospirillum de maíces de origen recientepor ancestros del mismo cereal. 2011-12-01 2016-05-02T20:54:39Z 2016-05-02T20:54:39Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://revistas.ucr.ac.cr/index.php/agromeso/article/view/8689 10.15517/am.v22i2.8689 http://hdl.handle.net/10669/17670 spa Copyright (c) 2014 Agronomía Mesoamericana application/pdf Universidad de Costa Rica Mesoamerican Journal of Agronomy; Agronomía Mesoamericana: Vol 22, No 2; 269-279 Agronomía Mesoamericana; Agronomía Mesoamericana: Vol 22, No 2; 269-279 2215-3608 1021-7444
institution Universidad de Costa Rica
collection Repositorio KERWA
language Español
description Theobjective of this work was to determine the affi nity andeffect of Azospirillum in corn. This bioassay was conductedin the state of Mexico, México, during the spring – summercycle of 2009. The root system of corn Palomero Toluqueño,Cacahuacintle, Cónico, Chalqueño and H-20, and of Teocintle,was collected, and after isolation and purifi cation ofstrains outstanding in their nitrogenasa activity (N’asa), werereinoculated aseptically in the same varieties at the seedlingstage (13 days) under hydroponic culture. Root volume wasdetermined, and also used to determine bacterial populationsand N’asa at 60 dde, in corn and Teocintle. Azospirillum didnot affect root volumen in relation to nitrogen fertilization,but the comparative study showed that strains isolated fromCónico and Chalqueño corn, in addition to Teocintle, formedmore root tissue in Chalqueño corn. Despite that the largestbacterial population was obtained with the strain isolatedfrom H-28 ans inoculated on corns Chalqueño, H-28 andCónico, the highest N’asa was recorded with strains isolatedfrom H-28, Palomero Toluqueño and Chalqueño, inoculatedon corn Chalqueño. There was a degree of affi nity, or effectof the homologous strains, between Azospirillum obtainedfrom corns H-28 and Chalqueño and their re-inoculation onthe same varieties, evaluating the number of azospirills andN’asa, and a survey of Azospirillum from recent corns, forancestors of the same cereal.
format Artículo
author Rangel Lucio, José Antonio
Rodríguez Mendoza, María De las Nieves
Ferrera Cerrato, Ronald
Castellanos Ramos, Javier Zaragoza
Ramírez Gama, Ana María
Alvarado Bárcenas, Estéfana
spellingShingle Rangel Lucio, José Antonio
Rodríguez Mendoza, María De las Nieves
Ferrera Cerrato, Ronald
Castellanos Ramos, Javier Zaragoza
Ramírez Gama, Ana María
Alvarado Bárcenas, Estéfana
Affinity and effect of Azospirillum sp. in corn.
author_sort Rangel Lucio, José Antonio
title Affinity and effect of Azospirillum sp. in corn.
title_short Affinity and effect of Azospirillum sp. in corn.
title_full Affinity and effect of Azospirillum sp. in corn.
title_fullStr Affinity and effect of Azospirillum sp. in corn.
title_full_unstemmed Affinity and effect of Azospirillum sp. in corn.
title_sort affinity and effect of azospirillum sp. in corn.
publisher Universidad de Costa Rica
publishDate 2011
url http://revistas.ucr.ac.cr/index.php/agromeso/article/view/8689
http://hdl.handle.net/10669/17670
_version_ 1605706400102612992
score 11.746185