Affinity and effect of Azospirillum sp. in corn.

Theobjective of this work was to determine the affi nity andeffect of Azospirillum in corn. This bioassay was conductedin the state of Mexico, México, during the spring – summercycle of 2009. The root system of corn Palomero Toluqueño,Cacahuacintle, Cónico, Chalqueño and H-20, and of Teocintle,was c...

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Autores Principales: Rangel Lucio, José Antonio, Rodríguez Mendoza, María De las Nieves, Ferrera Cerrato, Ronald, Castellanos Ramos, Javier Zaragoza, Ramírez Gama, Ana María, Alvarado Bárcenas, Estéfana
Formato: Artículo
Idioma: Español
Publicado: Universidad de Costa Rica 2011
Acceso en línea: http://revistas.ucr.ac.cr/index.php/agromeso/article/view/8689
http://hdl.handle.net/10669/17670
Sumario: Theobjective of this work was to determine the affi nity andeffect of Azospirillum in corn. This bioassay was conductedin the state of Mexico, México, during the spring – summercycle of 2009. The root system of corn Palomero Toluqueño,Cacahuacintle, Cónico, Chalqueño and H-20, and of Teocintle,was collected, and after isolation and purifi cation ofstrains outstanding in their nitrogenasa activity (N’asa), werereinoculated aseptically in the same varieties at the seedlingstage (13 days) under hydroponic culture. Root volume wasdetermined, and also used to determine bacterial populationsand N’asa at 60 dde, in corn and Teocintle. Azospirillum didnot affect root volumen in relation to nitrogen fertilization,but the comparative study showed that strains isolated fromCónico and Chalqueño corn, in addition to Teocintle, formedmore root tissue in Chalqueño corn. Despite that the largestbacterial population was obtained with the strain isolatedfrom H-28 ans inoculated on corns Chalqueño, H-28 andCónico, the highest N’asa was recorded with strains isolatedfrom H-28, Palomero Toluqueño and Chalqueño, inoculatedon corn Chalqueño. There was a degree of affi nity, or effectof the homologous strains, between Azospirillum obtainedfrom corns H-28 and Chalqueño and their re-inoculation onthe same varieties, evaluating the number of azospirills andN’asa, and a survey of Azospirillum from recent corns, forancestors of the same cereal.