FIS H analysis in Pisum sativum and P. fulvum with a telomeric sequence from Arabidopsis and a ribosomic one from soybean.

The sequence 18/25S rDNA from soybeanand the TTTAGGG from A. thaliana were used as probesto analyze the genomic organization of Pisum sativum, P.fulvum and a F4 line (P. sativum X P. fulvum) by fluorescentin situ hybridization (FISH). The experiment was conductedat the University of Córdoba, Spai n...

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Autores Principales: Bolaños Herrera, Alfredo, Cabrera Caballero, Adoración, Recio Castro, Rocío
Formato: Artículo
Idioma: Español
Publicado: Universidad de Costa Rica 2009
Acceso en línea: http://revistas.ucr.ac.cr/index.php/agromeso/article/view/11862
http://hdl.handle.net/10669/17510
Sumario: The sequence 18/25S rDNA from soybeanand the TTTAGGG from A. thaliana were used as probesto analyze the genomic organization of Pisum sativum, P.fulvum and a F4 line (P. sativum X P. fulvum) by fluorescentin situ hybridization (FISH). The experiment was conductedat the University of Córdoba, Spai n during the summer of2006. The probe 18/25S produced varia ble signals in thethree genotypes. These rDNA signals correspond to thenucleolar organizing pai rs (NOR `s) on chromosomes 4 and7 of P. sativum, and 4, 7 and 5 of P. fulvum. The intensityof signals varie d from very strong to moderately-strong in P.sativum to small and secrete in P. fulvum, suggesting cleardifferences on the number of times that the sequence 18/25Sis repea ted in the genome of these two specie s. Si gnalsobserved in the F4 line using the rDNA probe resembled P.sativum and P. fulvum knobs. Two NOR `s were observed onthe F4 line, although one of the lines showed very low signalsfor one pai r of chromosomes. The TTTAGGG sequencehybridized with the telomeres of the three lines, which showsthat the telomeric sequence from A. thaliana is also presentin the Pisum genera.