Molecular characterization using rep-PCR of native isolates of Bacillus thuringiensis, obtained from soil samples

Bacillus thuringiensis is a Gram-positive bacteria forming spores, which produces parasporal crystals of a proteic nature, toxic against various orders of insects and biodegradable and harmless to other species. This research use the experimental model, allowing, through observation techniques, micr...

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Main Authors: Galvis, Fabián, Yolima Moreno, Laura
Format: Artículo
Language: Español
Published: 2015
Subjects:
Online Access: http://revistas.ucr.ac.cr/index.php/agrocost/article/view/15207
http://hdl.handle.net/10669/14005
Summary: Bacillus thuringiensis is a Gram-positive bacteria forming spores, which produces parasporal crystals of a proteic nature, toxic against various orders of insects and biodegradable and harmless to other species. This research use the experimental model, allowing, through observation techniques, microbiological and biochemical identification of B. thuringiensis from soil samples from municipalities of Cúcuta, El Zulia, Los Patios, San Cayetano and Villa del Rosario, Norte de Santander, Colombia, and its subsequent characterization with molecular markers Bc-Rep and MB1. Microbiological and biochemical tests identified 10 isolates as B. thuringiensis; the results of phylogenetic analysis showed significant differences in the clusters obtained with Bc-Rep and MB1 markers. With Bc-Rep a low index of similarity (18%) was recorded, while with the marker MB1 a higher similarity index, 58%, was obtained. This work indicated a great genetic variability among isolates, showing that markers Bc-Rep and MB1 are highly effective to differentiate closely related strains, thus becoming a genetic tool of great value for studies of identification and diversity in B. thuringiensis.