Identificación molecular del hongo Colletotrichum spp., aislado de banano (Musa spp) de altura en la zona de Turrialba y determinación de su sensibilidad a fungicidas poscosecha

Molecular identification of the fungus Colletotrichum spp., isolated from banana (Musa spp) in high lands from Turrialba and determination of their sensitivity to postharvest fungicides. Anthracnose is one of the most serious banana post-harvest diseases. A total of 25 Colletotrichum isolates were o...

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Autores Principales: Salazar, Eduardo, Hernández, Ramses, Tapia, Ana María, Gómez Alpízar, Luis
Formato: Artículo
Idioma: Español
Publicado: 2015
Materias:
pcr
Acceso en línea: http://revistas.ucr.ac.cr/index.php/agrocost/article/view/9964
http://hdl.handle.net/10669/13942
Sumario: Molecular identification of the fungus Colletotrichum spp., isolated from banana (Musa spp) in high lands from Turrialba and determination of their sensitivity to postharvest fungicides. Anthracnose is one of the most serious banana post-harvest diseases. A total of 25 Colletotrichum isolates were obtained from banana fruits grown in 5 areas in Turrialba County, Costa Rica; 5 isolates per area. Isolates were characterized on the basis of polymerase chain reaction (PCR) using species- specific primers of the ITS region and ß-tubulin, gene, and their in vitro sensitivities to the fungicides thiabendazole, imazalil and myclobutanil at 0.1, 1.0, 10.0 y 100.0 μg.ml-1. All isolates were identified as Colletotrichum gloeosporioides and were sensitive to thiabendazole, whereas most isolates showed a marked reduction in sensitivity to imazalil and myclobutanil.