Direct somatic embryogenesis in Coffea arabica L. cvs. Caturra and Catua?: effect of tricontanol, light condition, and medium consistency

The influence of triacontanol (TRIA) concentration and its interaction with indole-3- acetic acid (IAA) on direct somatic embryogenesis (DSE) of Coffea arabica L. cvs Caturra and Catua? was determined. Additionally, light conditions and culture medium consistency (semisolid vs. liquid) were evaluate...

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Main Authors: Gatica Arias, Andr?s Mauricio, Arrieta Espinoza, Griselda, Espinoza Esquivel, Ana Mercedes
Format: Artículo
Language: Inglés
Published: 2015
Subjects:
Online Access: http://revistas.ucr.ac.cr/index.php/agrocost/article/view/6777
http://hdl.handle.net/10669/13847
Summary: The influence of triacontanol (TRIA) concentration and its interaction with indole-3- acetic acid (IAA) on direct somatic embryogenesis (DSE) of Coffea arabica L. cvs Caturra and Catua? was determined. Additionally, light conditions and culture medium consistency (semisolid vs. liquid) were evaluated. A higher average of somatic embryos per explant from Caturra (3.9?0.5) and Catua? (3.6?0.5) leaves was obtained when 4.55 ?M TRIA was added to the half-strength Murashige and Skoog (1962) medium supplemented with 1.1 ?M benzilaminopurine (BAP) and 2.85 ?M IAA. In relation to medium consistency, the highest number of embryoids in Caturra (3.2?0.2) and Catua? (6.0?0.4) explants was obtained using Yasuda et al. (1985) semisolid medium. Regarding photoperiod, the number of embryoids per explant obtained for Catua? cultured under a 16 h light photoperiod was 7.6?1.0 and in the dark 6.2?0.6. In Caturra, 4.2?0.4 embryoids were produced in the dark and 3.8?0.5 with 16 h light. No somatic embryos were observed in Caturra and Catua? explants after 12 weeks of culture in liquid Yasuda et al. (1985) medium, and under any of the light conditions.