Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization

artículo -- Universidad de Costa Rica. Escuela de Agronomía, 2014

Main Authors: Bolaños Villegas, Pablo Alberto, Yang, Xiaohui, Makaroff, Christopher A., Jauh, Guang-Yuh
Format: Artículo
Language: Inglés
Published: Bio-protocol 4(7), e1102, 2014 2015
Subjects:
Online Access: http://www.bio-protocol.org/e1102
http://hdl.handle.net/10669/11313
id RepoKERWA11313
recordtype dspace
spelling RepoKERWA113132018-06-13T15:58:27Z Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization Bolaños Villegas, Pablo Alberto Yang, Xiaohui Makaroff, Christopher A. Jauh, Guang-Yuh Meiosis Arabidopsis thaliana Cromosomas Hibridización in situ fluorescente artículo -- Universidad de Costa Rica. Escuela de Agronomía, 2014 This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes. This work was supported by research grants from Academia Sinica (Taiwan), the National Science and Technology Program for Agricultural Biotechnology (NSTP/AB, 098S0030055-AA, Taiwan) and the National Science Council (99-2321-B-001-036-MY3) to G-YJ, by a grant (MCB0718191) to CAM from the National Science Foundation (NSF) and by a study abroad contract to PBV from the University of Costa Rica. UCR::Vicerrectoría de Docencia::Ciencias Agroalimentarias::Facultad de Ciencias Agroalimentarias::Escuela de Agronomía 2015-02-24T21:43:55Z 2015-02-24T21:43:55Z 2014-04-05 info:eu-repo/semantics/article Artículo científico http://www.bio-protocol.org/e1102 http://hdl.handle.net/10669/11313 en_US application/pdf Bio-protocol 4(7), e1102, 2014
institution Universidad de Costa Rica
collection Repositorio KERWA
language Inglés
topic Meiosis
Arabidopsis thaliana
Cromosomas
Hibridización in situ fluorescente
spellingShingle Meiosis
Arabidopsis thaliana
Cromosomas
Hibridización in situ fluorescente
Bolaños Villegas, Pablo Alberto
Yang, Xiaohui
Makaroff, Christopher A.
Jauh, Guang-Yuh
Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
description artículo -- Universidad de Costa Rica. Escuela de Agronomía, 2014
format Artículo
author Bolaños Villegas, Pablo Alberto
Yang, Xiaohui
Makaroff, Christopher A.
Jauh, Guang-Yuh
author_sort Bolaños Villegas, Pablo Alberto
title Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
title_short Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
title_full Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
title_fullStr Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
title_full_unstemmed Protocol for the preparation of Arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
title_sort protocol for the preparation of arabiopsis meiotic chromosome spreads and fluorescent in situ hybridization
publisher Bio-protocol 4(7), e1102, 2014
publishDate 2015
url http://www.bio-protocol.org/e1102
http://hdl.handle.net/10669/11313
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score 11.800952